12. Monoclonal Antibody Production PDF

Title	12. Monoclonal Antibody Production
Author	Joyce Ann Magsakay
Course	IMMUNOLOGY AND SEROLOGY
Institution	Our Lady of Fatima University
Pages	1
File Size	54.6 KB
File Type	PDF
Total Downloads	38
Total Views	301

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Summary

IMMUNOLOGY SEROLOGY MONOCLONAL ANTIBODY PRODUCTION MONOCLONAL ANTIBODIES immunoglobulins derived from single plasma cells produced HYBRIDOMA TECHNOLOGY discovered GEORGES KOHLER CESAR MILSTEIN who used SENDAI VIRUS (an influenza) that characteristically causes cell fusion Uses: Initially used for di...

Description

IMMUNOLOGY & SEROLOGY

MONOCLONAL ANTIBODY PRODUCTION MONOCLONAL ANTIBODIES - immunoglobulins derived from single plasma cells - produced by HYBRIDOMA TECHNOLOGY - discovered by GEORGES KOHLER & CESAR MILSTEIN who used SENDAI VIRUS (an influenza) that characteristically causes cell fusion Uses:  Initially used for in-vitro diagnostic testing (Ex: Pregnancy testing)  Detection of tumor antigens & measurement of hormone levels  Recently used as therapeutic agents EXAMPLES OF MONOCLONAL ANTIBODIES MONOCLONAL ANTIBODY TARGET TREATMENT APPLICATION Trastuzumab (Herceptin) HER-2 / neu protein Metastatic Breast Cancer Rituximab (Rituxan) CD-20 on B-cells Non-Hodgkin’s Lymphoma Colorectal Cancer, Catuximab (Erbitux) EGFR Head & Neck Cancer Bavacizumab (Avastin) VEGF Colorectal, non-small PROCESS OF MONOCLONAL ANTIBODY PRODUCTION: Inject Ag to the Mouse

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Isolate/Harvest the Spleen cell

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Mix with Myeloma cell

Run in ELISA

JOYCE ANN S. MAGSAKAY

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Place in HAT medium

Get the supernatant

BS MEDICAL LABORATORY SCIENCE

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Add Polyethyline Glycol

* Spleen-Myeloma (Hybridoma) * Spleen-Spleen * Myeloma-Myeloma * Spleen only * Myeloma only

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OLFU VALENZUELA...