580164 Chemistry of Life p H and Buffers Q PDF

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Chemistry of Life: pH and Buffers

Student Name Rhyreef Patton Date

1

Dat Data a Activity 1 1. Calculate the volume of 1 M NaOH needed to prepare 10.0 mL of a 0.10 M solution. volume of 1 M NaOH is 1 ml. 2. Calculate the volume of 1 M HCl needed to prepare 10.0 mL of a 0.10 M solution.

Dat Data aT Table able 1

Initial pH

Water pH 4 buffer pH 6 buffer pH 8 buffer

pH after 1 drop 0.1M HCl

pH after 10 drops 0.1M HCl

pH after 1 drop 0.1M NaOH

pH after 10 drops 0.1M NaOH

6

6

6

6

6

5

5

4

5

6

7

8

8

8

10

6

6

5

6

6.5

3. In Activity 1, what happened to the pH of the water sample as 0.1 M HCl was added? How did this compare to what happened with addition of one drop of 0.1 M HCl to each buffer solution? Nothing changed with the water, but the buffers changed ph. 4. In Activity 1, why did the pH of the buffer solutions change after the addition of 10 drops of 0.1 M NaOH? Activity 2

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2 They changed because the concentration got higher

Activity 2 Dat Data aT Table able 2

Initial pH

pH after 1 drop 0.1M NaOH

pH after 2 drops 0.1M NaOH

pH after 4 drops 0.1M NaOH

pH after 8 drops 0.1M NaOH

100 %

6

6

6

6

6

50%

6

6

6

6

7

25%

6

6

6

8

7

12.5 %

6

6

7

7

9

5. In Activity 2, which concentration was the effective buffer? Explain what happened chemically as the buffer became more dilute.

6. Explain how the buffer could be diluted (in Activity 2), yet maintain the same pH. Hint: Reference the Henderson-Hasselbalch equation.

Photos Photo 1 Insert photo of results of Activity 1. The following should be visible in this photo:  Row and column labels

© 2016 Carolina Biological Supply Company

3

Photo 2 Insert photo of results of Activity 2. The following should be visible in this photo:  Row and column labels

© 2016 Carolina Biological Supply Company

4

© 2016 Carolina Biological Supply Company...