Bacterial smear Quizlet PDF

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6/24/2021

Exercise 12 Flashcards | Quizlet

Exercise 12 Terms in this set (5)

Identify the steps taken in the preparation of a bacterial smear to prevent contamination of the culture and the preparer of the smear

Clean slide throughly heat fix Flame loop remove cap flame the tube place loop inside tube and get bacterial culture out flame tube again close the tube and then take loop and put the bacteria on the slide and then flame loop again keep work station clean

Discuss possible outcomes if

Bacteria could still be alive, stain could not have

an air-dried smear were not

been well applied, cells could be washed off when

heat-fixed before proceeding

stained

with the staining step

For slant preparation, what will

If there is too much water, it may be harder to read

happen if too large drop

because the specimens will be too spread out

oswater was put on the slide?

What might happen if a slide

Overheating will denature and rupture the cell wall

were overheated during the heat-fixing step?

After a smear has been stained,

Thin, hazy smears are better because a thick smear

do you believe a thin, hazy

will diminish the amount of light that can pass

smear or a thick, dense smear

through making it difficult to visualize the

will make the best preparation

morphology of single cells under the microscope

for microscope examination?

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