BIO150 LAB Report PDF

Preview

Summary

FAKULTI SAINS GUNAANUNIVERISITI TEKNOLOGI MARACAWANGAN PERAK KAMPUS TAPAHBIO150 – METABOLISM & CELL DIVISIONSCIENTIFIC LAB REPORTNAME:STUDENT ID:GROUP:EXPERIMENT TITLE: ENZYME I – THE FASTEST ENZYME: CATALASELECTURER:DATE OF SUBMISSION:------------------------------------------------------------...

Description

FAKULTI SAINS GUNAAN UNIVERISITI TEKNOLOGI MARA CAWANGAN PERAK KAMPUS TAPAH BIO150 – METABOLISM & CELL DIVISION

SCIENTIFIC LAB REPORT NAME: STUDENT ID: GROUP: EXPERIMENT TITLE: ENZYME I – THE FASTEST ENZYME: CATALASE LECTURER:

DATE OF SUBMISSION: --------------------------------------------------------------------------------------------------------------------------Declaration of Academic Honesty Academic honesty or academic integrity is a very important virtue that all students should uphold at all times. I declare that the lab report submitted is not plagiarised and is entirely my own work, and that no part of it has been copied from any work produced by other person(s)/ source(s) or provided by any other student(s). I understand that issuing a false declaration can result in severe penalties and I am willing to be penalized if any form of copying found valid.

INTRODUCTION

Enzymes are the catalysts of biochemical reaction that speed up the reaction but stay unchanged at the end of the reaction (Rafida Razali, 2020). Catalase is an enzyme that is often found in the liver that catalyses the decomposition of hydrogen peroxide as it’s enzyme, to water and oxygen. It protects cellular organelles and tissue from damage by the peroxide. Liver, kidney and potato have a specific enzyme called catalase respectively. (Adam Augustyn, 2017)

This catalyst started a reaction and makes it faster as the rate of reaction increase. Each chemical reaction needs a minimum amount of energy, known as the activation energy to make the it happen. The lower the activation of energy of a reaction, the faster it takes place. And if the activation energy is too high, the reaction may does not occur at all. (Svenja Lohner, 2016)

The catalase enzyme breaks down hydrogen peroxide (H2O2) into water and oxygen. 2H2O2 ----catalase--

2H2O2 + O2

There are three objectives in this experiment :

1. To investigate the reaction of catalase (hydrogen peroxide) on these different samples. 2. To analyse the factors that effects the reaction of catalase.

The hypothesis in this experiment is that the smaller the surface area of the sample, the higher the presence of oxygen. The manipulated variable is the surface area of the sample. The responding variable is the presence of oxygen. The constant variable is the amount of hydrogen peroxide. The problem statement is what is the factor that affect the rate of the reaction of the enzymes to produce oxygen. Therefore, there were samples that have been chosen by different sizes and temperature to find the factor in change of the rate of reaction in this experiment.

APPARATUS & MATERIALS

1.

18 ml Hydrogen peroxide

2.

Liver

3.

Kidney

4.

Potato

5.

9 test tubes

6.

Splints

7.

Bunsen burner

8.

Pestle and mortar

9.

Dropper

10. Wire gauze 11. Beaker 12. Forceps 13. Spatula 14. Assorted rubber stopper

PROCEDURE 1.

2 ml hydrogen peroxide was poured into each of the test tubes.

2.

1 cm of liver was cut & dropped into the test tube of hydrogen peroxide.

3.

The test tube was shaken and was observed & recorded.

4.

Then, the presence of oxygen was tested by a splint by putting it into the test tube for a while.

5.

Another 1cm cube of liver was taken and grinded. It was transferred into the test tube hydrogen peroxide.

6.

The test tube was shaken slowly and was observed and recorded then compared to the cube of liver.

7.

Another cube of liver was taken and boiled for 3 minutes.

8.

It was then dropped into the test tube of 2ml hydrogen peroxide then was shaken well.

9.

A splint was used as well to test the presence of oxygen and the observations was recorded.

10. Step 2 until 6 were repeated by replacing liver with kidney and potato. 11. All observations were recorded in the table below.

RESULTS

TABLE 1: CATALASE REACTION Test tube containing

Observations

Presence of Oxygen

Cube of liver

A little bubble formed on the surface

Oxygen is presence

Ground liver

More bubbles formed on the surface

Oxygen is presence

Boiled liver

No bubbles formed on the surface

Oxygen is absent

Cube of kidney

A little bubbles formed on the surface

Oxygen is presence

Ground kidney

More bubbles formed on the surface

Oxygen is presence

Cube of potato

A little bubbles formed on the surface

Oxygen is presence

Ground

More bubbles formed on the surface

Oxygen is presence

potato

DISCUSSION

When there were bubbles formed from the reaction, it showed the presence of oxygen. The more the bubbles, the higher the oxygen presence.

The cubes of liver, kidney and potato showed a little bubbles on the surface compared to when they were grounded. This is due to the smaller surface area of the samples, where the enzymes react faster to produce oxygen and formed more bubbles on the surface. Boiled liver showed no bubbles on the surface as enzymes works at an optimum temperature, 37°C-39°C, and when it reached above it the enzyme will stop working and started to break down.

CONCLUSION The stated hypothesis is accepted. The results showed the most bubbles formed for the grounded samples and lesser bubbles for the cubed samples as the surface area of grounded samples are smaller than the cubed samples.

REFERENCES

1) Rafida Razali. (2020). BIO150: Metabolism and Cell Division, Enzyme [PowerPoint slides]. Accessed 25 April from UiTM, N.D. 2) Adam Augustyn. (2016). Catalase, Function & Applications. Accessed 24 April 2021 from https://www.britannica.com/science/catalase 3) D. Roston, Q. Cui. (2016). Enzymology in Methods in Enzymology. Accessed 24 April 2021

from

https://www.sciencedirect.com/topics/medicine-

anddentistry/enzymology#:~:text=Enzymology%20is%20the%20branch%20of,fold%2 0int o%20their%20native%20state. 4) Svenja Lohner. (2016). Exploring Enzymes. Acceseed 25 April 2021 from https://www.scientificamerican.com/article/exploring-enzymes/ 5) Biology - Lab 6 Enzyme Catalysis Video. (2020, November 11). [Video]. YouTube. Accessed 23 April 2021 from https://www.youtube.com/watch?v=_ViCY7BycbM 6) Education.com Organization. (2013). Catalase and Hydrogen Peroxide Experiment. Accessed 24 April 2021 from https://www.education.com/science-fair/article/activator/...