BIOL+3P28+2020+Syllabus PDF

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BIOL 3P28 Developmental Biology September 2020 Lecture:

Tuesday & Friday 12:30 – 2:00 pm ONLINE

Instructor:

Dr. Amanda Lepp [email protected] Fridays 10-11am online or email any inquiries

Teaching Assistants: Jacob Lee [email protected] Michael Tolentino [email protected] Mikael Morneault [email protected] Senior Laboratory Instructor:

John Ciolfi e-mail: [email protected]

Laboratories:

Lab Exercises to be posted on Mondays as indicated in this course outline. Online Lab Material will begin posting on September 28, 2020. See Lab Schedule posting and due dates. Labs are ASYNC. LAB SECTION 1 - M 2:00 – 5:00 LAB SECTION 2 - T 2:00 – 5:00 LAB SECTION 3 - W 2:00 – 5:00 LAB SECTION 4 – T 6:00 – 9:00 LAB SECTION 5 – R 2:00 – 5:00 Labs will run on alternate weeks beginning the week of Sept 28, 2020.

Course Calendar Description: Principles of vertebrate and invertebrate development. Topics include life cycles and the evolution of developmental patterns; the genetic core of development; differential gene expression; cell-cell communication; fertilization; early development and axial specification in the selected vertebrates and invertebrates. Prerequisite: BIOL 2P02 and BIOL 2P03

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Required Textbook: “Developmental Biology” (12th edition) by Gilbert & Barresi, Published by Sinauer Laboratory Manual: Available on Sakai. A compilation of exercises some modified from Merriam, R. F. 1988, Experiments in Animal Development, Sinauer Associates, Sunderland, Mass. Course Communications: In the event of class cancellations or other important announcements, students will be informed through Sakai. Students are responsible for checking their email and Sakai regularly throughout the term.

Evaluation Components and Due Dates Assignment Test 1 Test 2 Test 3 Labs Labster Simulations Lecture Participation Research Proposal

Grade Weight 10% 20% 20% 20% 10% 10%

Due Date October 6th November 10th December 8th Throughout Throughout Throughout

10%

November 20th

Biology 3P28 Lecture Tentative Topic Schedule Note: adjustments may be made over the duration of the course Week Date Schedule & Suggested Readings 1 2 3 4

Sept 11th Sept 15th and 18th Sept 22nd and 25th Sept 29th and Oct 2nd

Intro/Developmental Organization (Ch 1) Specification and Patterning (Ch 2) Developmental Genetics (Ch 3) Cell to Cell Communication (Ch 4) Labs Begin.

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Oct 6th and 9th

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Oct 20th and 23rd

Stem Cells (Ch 5) Test #1 (Oct 6th) Reading week Sex Determination and Gametogenesis (Ch 6)

7 8 9 10 11 12 13

Oct 27th and 30th Nov 3rd and 6th Nov 10th and 13th Nov 17th and 20th Nov 24th and 27th Dec 1st and 4th Dec 8th

Fertilization (Ch 7) Axial Specification in Drosophila (Ch 9) Early Development Invertes (Ch 8/10) Test #2 (Nov 10th) Early Development Amphibians (Ch 11) Early Development Fish and Birds (Ch 11/12) Early Development Mammals (Ch 12) Test #3 2

Participation (10 marks): While attendance is not officially recorded during lectures, it is strongly encouraged, as it increases the opportunity for learning and student success. Each video lecture will be posted on Tuesdays and Fridays by 12:30pm. You will have 24 hours after the lecture is posted to complete that lectures’s “participation quiz” on sakai (so Wednesday and Saturday at 12:30pm). Questions will be asked throughout the video lecture- but will not be named on sakai (ie. Question 1, question 2) and you will have 24 hours to view the lecture, go online and answer them. Participation marks will accumulate throughout the term, and at the end of term these marks will be converted to a final grade out of 10. Students with a participation mark ≥ 8/10 will receive a full 10% towards their final grade. Students with participation marks between 5 and 7.9 out of 10 will receive that mark for clicker participation with no adjustments. Importantly, students who consistently fail to attend class and participate via clicker and score less than a 5/10 will receive a clicker grade of zero (0/10). Participation will officially begin with the first class of the term (September 11th, 2020). Research Proposal (10 marks): Each student will write a research proposal on a topic of their choosing. This proposal must be on one of the topics covered in the course. It is due on Nov. 20th by 4 pm via electronic copy submission through the assignment tab on Sakai. More details will be posted on Sakai. Lab Simulations: In an attempt to recreate these labs without the hands on aspect of physically being in a lab we will be using lab simulations by Labster. You must purchase a Labster voucher for this course from the bookstore ($39.75). NOTE: if you are in multiple courses using this software it does need to be purchased for each course. There will be a simulation (or two) assigned every other week. You will have 7 days to complete the assigned simulation(s). These simulations will make up 10% of your final grade, 5% for completing all simulations, 5% for your score on the participation quizzes in each simulation. Lab Schedule: Labs will run on alternate weeks beginning the week of Sept 28, 2020. Lab Material will be posted on Mondays. Lab reports will be due the following Monday. SEND YOUR LAB REPORTS BY EMAIL TO YOUR ASSIGNED TEACHING ASSISTANT (SEE ANNOUNCEMENTS TO IDENTIFY YOUR TA). DO NOT POST YOUR REPORTS ON SAKAI or SEND THEM TO J. CIOLFI or Dr. LEPP. LAB 1. How Slime Mold Amoebae Communicate to Promote Aggregation POSTED ON MONDAY SEPT 28. DUE NO LATER THAN 12 PM (NOON) ON MONDAY OCT 5. Week of Oct 12 No Lab this Week – Thanksgiving and Fall Reading Week LAB 2. Retinoids and Planarian Regeneration POSTED ON MONDAY OCT 19. DUE NO LATER THAN 12 PM (NOON) ON MONDAY OCT 26.

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LAB 3. Cleavage in Xenopus Eggs POSTED ON MONDAY NOV 2. DUE NO LATER THAN 12 PM (NOON) ON MONDAY NOV 9. LAB 4. Luxury Protein Synthesis in Differentiation (Chick Embryo) POSTED ON MONDAY NOV 16. DUE NO LATER THAN 12 PM (NOON) ON MONDAY NOV 23. NO LATE LABS WILL BE ACCEPTED WITHOUT A MEDICAL CERTIFICATE. ALL LATE LABS WILL RECEIVE A GRADE OF ZERO ‘0’. GENERAL LAB RULES & PROCEDURES (As outlined in the Brock University ‘Safety in the Sciences Manual.’) ALTHOUGH YOU WILL NOT BE ATTENDING THE LAB IT REMAINS IMPORTANT THAT YOU UNDERSTAND THE SAFETY RULES GOVERNING LAB CONDUCT. FOR THIS TERM, THESE RULES ARE FOR YOUR INFORMATION ONLY. 1. Be prepared! Know the safety rules and procedures that apply to the work that is being done. Determine the potential hazards, appropriate safety precautions and proper waste disposal techniques well in advance. 2. Know the location and proper use of emergency equipment, for example, safety showers, eye wash stations, fire blankets, fire extinguishers, first aid kits and defibrillators, and be familiar with emergency procedures, such as exits, alarm stations, emergency phones, and evacuation. 3. CSA rated safety goggles must be worn in all areas where a splash hazard exists, generally where chemicals are used, handled or stored, or alternate appropriate eye protections where particular hazards exist, e.g., UV light, lasers, particulate matter or systems under pressure. 4. Protective clothing should be worn as appropriate to the work being done. Long hair and loose clothing must be confined; shorts are not permitted in labs without lab coats. 5. Proper protective footwear is required; sandals or high heels should not be worn. 6. Horseplay and pranks are expressly forbidden as outlined by the OHSA. 7. Eating, drinking, smoking or applying cosmetics are not permitted in any lab or chemical storage area. 8. Mouth pipetting is absolutely forbidden.

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9. All bottles, flasks and vials containing chemicals, including wastes, must be labeled (with information about contents, concentration, date and individual(s) involved). The only exceptions are chemicals that will be consumed in one lab session by one individual. 10. Chemicals from unlabelled containers are not to be used and their presence shall be reported to the lab supervisor. 11. Suitable warning signs must be posted when hazardous situations are present, such as the use of highly toxic chemicals, radioactive substances, biohazardous materials, work involving high pressure, X-rays or lasers. 12. All work areas shall be kept clean, free from obstruction and not pose any danger to individuals. 13. Access to exits and emergency equipment should never be blocked. 14. Spilled chemicals should be cleaned up immediately and disposed of properly. A separate labeled container is to be used for broken glassware. 15. Equipment shall be properly maintained, and used only for its designed purpose. Access to electrical connections and moving parts should be guarded. 16. All accidents will be promptly reported to the lab or activity supervisor, who will administer first aid and/or arrange for further medical attention as well as complete an Injury/Incident Report Form (www.brocku.ca/oehs/forms) 17. Undergraduate students must not work unsupervised in a laboratory. 18. Avoid working alone in a laboratory. If it seems necessary, discuss hazard levels and monitoring requirements with supervisor. If unavoidable, institute a buddy system or notify Campus Security Services (ext. 3200) when arriving or departing. 19. Labs, storerooms and high-risk equipment must be locked or tagged when unattended. 20. Always thoroughly wash hands with soap and water before leaving the work area. This applies even if one has been wearing protective gloves. 21. Proper, responsible, waste disposal practices must be used at all times, for example, identification classification and registration of waste. 22. Turn off all cell phones, pagers and electronic devices before entering the laboratory.

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BIOHAZARD SAFETY RULES ALTHOUGH YOU WILL NOT BE ATTENDING THE LAB IT REMAINS IMPORTANT THAT YOU UNDERSTAND THE SAFETY RULES GOVERNING LAB CONDUCT. FOR THIS TERM, THESE RULES ARE FOR YOUR INFORMATION ONLY. 1. Know the microorganisms that you will be working with in the lab. 2. Backpacks, bags, and coats are not allowed in the Laboratory room. Do not bring them in with you. There are chains and hooks provided in the hallway. Valuable items (purses, laptops, iPads, and phones) may be immediately placed and kept stored in the microscope cabinet at your bench. The only items allowed on the bench top is the lab protocol and spare paper for notes. 3. A pen and pencil will be provided in the lab and must remain in the lab. Do not put writing utensils in your mouth. 4. You must bring and wear a Lab Coat and safety glasses for all Labs (stored in a separate bag when not in use). 5. You must wear closed toed shoes. 6. Gloves will be provided. 7. Long hair must be in a ponytail or fastened away from face. Avoid touching face and body. 8. No food or beverages are allowed in the lab. 9. Do not use earphones with cell phones, I-pods or MP3 players during the lab time, as it is a safety hazard. 10. Nothing is to be poured down the drain; all waste must go into the appropriate labeled waste containers. 11. Always wipe bench top down with 70% ethanol at the beginning and end of your lab. 12. Never leave a flame unattended. 13. Inform your TA of a spill right away. 14. Wash hand with soap and water before you leave the lab.

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Academic Policies Late Submission Policy: Late submission of assigned coursework will not be accepted, unless accompanied by medical documentation. Please see the medical exemption policy and medical health certificate at http://www.brocku.ca/health-services/policies/exemption Makeup Exams: One makeup examination will be provided for each test for students with medical documentation only. In order to write the makeup exam, you must notify Dr. Lepp of a medical exemption by email prior to missing a test. Original copies of medical documentation must be provided as soon as possible. If documentation has not been provided within one week of missing a test or assignment, a grade of zero may be given. Academic Integrity: Academic misconduct is a serious offence. The principle of academic integrity, particularly of doing one’s own work, documenting properly (including use of quotation marks, appropriate paraphrasing and referencing/citation), collaborating appropriately, and avoiding misrepresentation, is a core principle in university study. Students should consult Section VII, “Academic Misconduct”, in the “Academic Regulations and University Polices” entry in the Undergraduate Calendar, available at http://brocku.ca/webcal to view a fuller description of prohibited actions, and the procedures and penalties. Plagiarism software: This course may use Turnitin.com, phrase-matching software. If you object to uploading your assignments to Turnitin.com for any reason, please notify the instructor to discuss alternative submissions. Academic Accommodation: As part of Brock University's commitment to a respectful work and learning environment, the University will make every reasonable effort to accommodate all members of the university community with disabilities. If you require academic accommodations related to a documented disability to participate in this course, you are encouraged to contact Services for Students with Disabilities in the Student Development Centre (4th floor Schmon Tower, ex. 3240). You are also encouraged to discuss any accommodations with the instructor well in advance of due dates and scheduled assessments. Academic Accommodation due to Religious Obligations: Brock University acknowledges the pluralistic nature of the undergraduate and graduate communities such that accommodations will be made for students who, by reason of religious obligation, must miss an examination, test, assignment deadline, laboratory or other compulsory academic event. Students requesting academic accommodation on the basis of

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religious obligation should make a formal, written request to their instructor(s) for alternative dates and/or means of satisfying requirements. Medical Exemption Policy: The University requires that a student be medically examined in Health Services, or by an offcampus physician, prior to an absence due to medical reasons from an exam, lab, test, quiz, seminar, assignment, etc. A completed Brock University medical certificate must be provided for missed tests or assignments. The medical certificate can be found at http://www.brocku.ca/health-services/policies/exemption

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