Biotechnology Lab Worksheet PDF

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NAME: DATE: 04/13/19 Virtual Lab #13: Biotechnology 1. Open the 1st Virtual Lab: Reproductive cloning (http://learn.genetics.utah.edu/content/cloning/) 2. First, click the link labeled “What is cloning?” Read the provided information and watch the two videos: “Artificial Embryo Twinning” and “Somatic Cell Nuclear Transfer.” 3. What is cloning? It is simply asexual reproduction; genetically identical individuals that are independent

4. In one type of cloning technique, the cells of an embryo are separated into individual cells, each of which is allowed to grow and develop in a petri dish. Eventually each cell develops into a new, genetically identical embryo which can be implanted into a surrogate mother. What is this technique? a. Natural Twinning b. Artificial Embryo Twinning c. Gene Cloning d. Somatic Cell Nuclear Transfer e. All of the above 5. In a different cloning technique, the nucleus is removed from any cell of an animal’s body (e.g. a liver cell). The nucleus is transferred into a egg cell which has had its nucleus removed. This new cell is allowed to develop into an embryo that is implanted into a surrogate mother. What is this technique? a. Natural Twinning b. Artificial Embryo Twinning c. Gene Cloning d. Somatic Cell Nuclear Transfer e. All of the above 6. Click the back button to go back to where we started this lab. This time, click the link labeled “Click and Clone.” In this first part of the lab, you will use Somatic Cell Nuclear Transfer to clone the mouse “MiMi.” 7. Which of the following correctly matched the mouse with its role in the experiment? a. MiMi (brown) = surrogate mother b. Megdo (black) = surrogate mother c. Megdo (black) = mouse we will clone d. Megdo (black) = egg cell donor e. MiMi (brown) = egg cell donor

8. Follow the interactive through the six steps of the experiment. 9. Which cell has its nucleus removed and thrown away? a. The somatic cell b. The egg cell 10. What happens to the nucleus of the somatic cell? It is separated from the cell and placed into the egg cell that also has the nucleus removed

11. What color is the new baby mouse produced in this experiment? Brown 12. The new baby mouse is genetically identical to which of her “mothers?” a. Mimi, the somatic cell donor b. Megdo, the egg cell donor c. Momi, the surrogate mother d. The new mouse has a mixture of genes from all three of these mice e. None of the above 13. This same technique was used to create Dolly the sheep, the first cloned mammal, in 1996. Dolly was developed from an egg cell taken from a Scottish Blackface ewe (female sheep) and the nucleus of a mammary cell from a white Finn-Dorset ewe. Her surrogate mother was another Scottish blackface ewe. What type of sheep was Dolly? a. Scottish Blackface b. White Finn-Dorset c. A mixture of Scottish Blackface and white Finn-Dorset d. It’s impossible to tell given this information. 14. Open the 2nd Virtual lab: “Genetically Modified Organisms.” (http://www.pbs.org/wgbh/harvest/exist/) 15. Read through the arguments for and against the creation of genetically modified (GM) crops. At the bottom of each page, you will be asked if you think we should grow GM crops. Answer honestly. After the final page, you will be given the option to read all 12 arguments for and against growing GM crops (six arguments in favor of it and six arguments opposed). Make sure you read ALL TWELVE arguments. 16. Which of the following is NOT an argument AGAINST the use of genetically modified foods? a. GM foods might be harmful to people with allergies b. GM seeds could be too expensive for small farmers to buy and grow c. GM crops could help insects become resistant to pesticides

d. GM crops are over-regulated and have too much oversight from the corporations that produce them 17. Which of the following is NOT an argument FOR the use of genetically modified foods? a. GM crops are healthier than traditional crops and can help cure world-wide malnutrition b. GM crops can help farmers avoid many of the problems that currently affect their production (drought, insects, microbial infection, etc) c. The use of GM crops means more chemicals (fertilizers and herbicides) will be used on farms around the world d. The science behind GM crops is the same type of genetic modification that has been occurring naturally for a millennia. e. GM crops can help feed the poor and starving of the world. 18. Scroll back up to the top of the page. On the right side of the screen you will see a small tan box containing five links. Click the link labeled “What’s for dinner?” On the new page, click the image on the right side to open the “Guess What’s Coming for Dinner?” activity. 19. Click all 10 of the items on the table, and read the provided information about each. 20. Which of the following is NOT an organism scientists are currently trying to genetically modify (or have already genetically modified)? a. Bananas b. Petunias c. Flies d. Cotton e. Apples f. Fish g. None of the above 21. Why are scientists trying to create genetically modified bananas? To use them as edible vaccines

22. Why are scientists trying to genetically modify salmon? a. To make them reproduce faster b. To make them taste better c. To make them grow faster d. To make the meat last longer in the freezer 23. Why are scientists trying to genetically modify cotton? To make it moth and drought resistant...