Differential selective analysis PDF

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Summary

selective and differential ingredient assignment got an A+...

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Special Media for Isolating Bacteria Analysis 1) This table represents the composition of MSA and MAC plates compared to plain nutrient agar. Using the information from the videos and the Lab 4 introduction, highlight for Mannitol salt agar and for MacConkey agar the selective  ingredient in green and the differential ingredient in yellow, some ingredients are just there to support growth and will not be either. For 1 liter of media

Nutrient Agar

Mannitol Salt Agar

MacConkey Agar

Peptone

20 gr

20 gr

20 gr

NaCl

5 gr

75gr

5 gr

Bile Salts Agar Mannitol

5 gr 15 gr

15 gr 10 gr

Lactose Phenol Red

15 gr

10 gr 0.025 gr

Neutral Red

0.03 gr

Crystal Violet

0.001 gr

2) These are your plate results obtained following the direction in Lab 4 introduction and method:

Complete the Table below using the above results: Organism

Mannitol Salt Agar

MacConkey Agar

Growth Yes or No

Color

Growth Yes or No

Color

Escherichia coli

no

yes

pink

Staphylococcus aureus Experimental sample

yes

no color was developed yellow

yes

pink

yes

no pink

3) Answer the following questions: - Did you get the results that you expected for your control plates? (yes or no) E. coli MacConkey plate

____yes______

E. coli MSA plate

____no______

S. aureus MacConkey plate

_____no_____

S. aureus MSA plate

__yes________

- Did you get mannitol fermentation in your experimental sample MSA plate? (yes or no)______yes_______ - Did you get lactose fermentation in your experimental sample Mac plate? (yes or no) _________yes____ - Using the information from Lab 4 differential and selective media introduction, give the name (s) of 2 microorganisms that could be present in your experimental sample. ___________________S.aureus-mannitol salt agar______________  and ____________________________E.coli-_MacConkey plate______ 4) Many different media are used to differentiate and select bacteria. Blood Agar plates are often used in microbiology diagnostic as they allow hemolytic reactions to be observed on plates. Explore the sheep blood agar plate http://learn.chm.msu.edu/vibl/content/differential.html (Click to open the module) and answer the following questions A. Sheep blood agar plates are differential, selective or both? sheep blood agar is differential and enriched. B. What kind of hemolysis is depicted in the figure below? ___beta___________________________ C. Give the name of a bacteria that could give similar results ___alpha_________________________...