Title | Experiment 6 Paper chromatography of inks and dyes |
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Course | Chemistry |
Institution | High School - USA |
Pages | 4 |
File Size | 163.1 KB |
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Lab report on Paper chromatography of inks and dyes...
Experiment 6: Paper chromatography of inks and dyes Date of experiment: 10/22/19 Submission date: 9/24/19 Section: 010 TA: Rezoanul Islam Name:
Introduction:
In this experiment, Paper chromatography was used to separate the pigments in inks and dyes. Paper chromatography is usually the technique used to separate any substances that have colored dyes since the solvent on the paper evaporates, leaving
just the colored molecules behind to examine. It is for this reason that paper chromatography was the technique used for this lab since it would the dyes contained in permanent markers and water soluble pens that would be examined.
Experiment: Data: Water soluble pens
Spot 1
Distance from baseline to spot
7.2cm
Distance from baseline to edge of solvent
8.0cm
Rf Value
0.9
Calculation of Rf value: 7.2/8= 0.9 (Distance from baseline to spot divided by Distance from baseline to edge of solvent)
Permanent marker/alcohol soluble
Spot 1
Distance from baseline to spot
4.4 cm
Distance from baseline to edge of solvent
5.6 cm
Rf value
0.79
Rf calculation: 4.4 / 5.6 = 0.79 (Distance from baseline to spot divided by Distance from baseline to edge of solvent)
Water soluble pen chromatography paper
Permanent Marker chromatography paper
Conclusion: The results of this experiment found that the dyes in the water soluble pen travelled farther than the dyes in the permanent marker, which is corroborated by the fact that the water soluble dye had the higher Rf value. This means that the dyes found in the water soluble pen were more polar since water was a polar solvent and the dyes
travelled up the chromatography paper fairly quickly and had some actual pigment separation. The dyes in the water soluble pen and the permanent marker are clearly not the same as the dyes in the permanent marker took a while to travel up the paper and did not make it as far the water soluble pen.
Reference: 1. Department of Chemistry CHEM 2071 Organic Chemistry 1 Laboratory Manual, 2019 Edition; Macmillan learning Curriculum Solutions: Plymouth, MI; pp 41-42 2. “Paper Chromatography for Students.” Journal of Chemical Education, pubs.acs.org/doi/abs/10.1021/ed030p614....