Grossing and Specimen Receiving PDF

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Summary

MLSC-3230, Microanatomy and HistotechnologyStandard Routine Histology Workflow- Tissue is received in the pathology lab and then transferred into labelled blocks to be sent to the tissue processor room. - Tissues blocks are then placed into the tissue processor. After processing the tissue blocks go...

Description

MLSC-3230, Microanatomy and Histotechnology Standard Routine Histology Workflow -

Tissue is received in the pathology lab and then transferred into labelled blocks to be sent to the tissue processor room. Tissues blocks are then placed into the tissue processor. After processing the tissue blocks go to the embedding station. After embedding the tissue goes to the microtomy station. Prepared slides from this station then go onto be stained and cover slipped. Stained slides are put in a slide folder and sent to the pathologist for viewing and diagnosis.

Specimens Received in the Lab -

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Fresh samples are sent down unfixed for a rapid diagnosis. These specimens are potentially biohazardous since it is not fixed. These sample also cannot sit out for too long or else they will dry out and decay. More commonly, specimens are received fixed in formalin containers. These specimens should be in a volume of fixative that is 15 to 20 times its own volume.

Specimen Accessioning -

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The requisition is compared with the data on the specimen (patient number, DOB). Once the specimens have been matched with their requisitions, they are entered into the lab for processing one at a time. Close attention is paid to the specimen’s matching requisition to avoid any discrepancies and misdiagnoses. Specimens cannot be thrown out because surgical specimens are limited and cannot be recollected. Once the patient identifiers are prepared and matched, the specimen is entered into the LIS for registration and processing.

Grossing -

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Defined as macroscopic examination of the specimen, taking note of the physical features and dimensions of the specimen. Measurements, consistency, colour weight and shape are typically recorded. Grossing in the grossing room is done with a scalpel on a cutting board in a wellventilated environment. Forceps and scissors are also used for dissection. Once dissected, the specimen pieces are placed into cassettes. Specimens can come in all shapes and sizes. Core biopsies are collected through surgical needles and produces a long tube of tissue. Curetting’s or scrapings of tissues can also be used. Tiny biopsies are placed on sponges within the cassettes to avoid any pieces falling through the cassette holes. Specimens may be received oriented to clarify the specimens placement in the patient. The number of cassettes and pieces of specimen per cassette are documented....