Hematology Reviewer: Formulas, Reference Values, Equipment & Instruments, and Additional Notes PDF

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1Medical Laboratory Science Study Notes in CLINICAL HEMATOLOGY A.M. Tamdang, A. Tomaro, V. Villapa, S Yabes ( December 22, 2021)HEMATOLOGY ADDITIONAL NOTESFormulas used in HematologySummary of Reference RangesHematology Equipment and InstrumentsMust Know in HematologyFORMULAS USED IN HEMATOLOGYManua...

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Notre Dame of Marbel University Medical Technology Department Medical Laboratory Science Study Notes in CLINICAL HEMATOLOGY A.M.J. Tamdang, A.M. Tomaro, V.C. Villapa, S.Y Yabes ( December 22, 2021)

HEMATOLOGY ADDITIONAL NOTES Formulas used in Hematology Summary of Reference Ranges Hematology Equipment and Instruments Must Know in Hematology

FORMULAS USED IN HEMATOLOGY Manual Cell Count Formula including RBCs and WBCs Total Count = cells counted x dilution factor x 10 / area WBC Count Correction WBC Count Correction = uncorrected WBC count x 100 / number of nRBCs per 100 WBCs +100 Mean Cell Volume (MCV) Formula = fL MCV = Hct (%) x 10 / RBC count x 1012/L) = fL Mean Cell Hemoglobin (MCH) Formula = pg MCH = Hgb (g/dL) x 10 / RBC count (x1012/L) = pg Mean Cell Hemoglobin Concentration (MCHC) Formula - g/dL MCHC = Hgb (g/dL) x 100 / Hct (%) = g/dL Reticulocytes Formula % Reticulocytes = number of reticulocytes. x 100 / per 1000 (RBC counted)

Nucleated RBCs NRBC = uncorrected WBC count x 100 / (NRBS per100WBC) + 100 Total Cell Count Total cell count = cells counted x dilution factor / area (mm2) x depth (0.1) Total count = cells counted x dilution factor x 10 / area (mm2) WBC count WBC count = cells counted x dilution factor / area counted (mm2) x depth Platelet count Platelet count (per mL) = No. of cells counted / volume of the chamber (uL) x dilution x 1000 Platelet Estimate (Average no. of platelets/ field) x total RBC count / (200 RBCs/ field) International normalized ratio INR = (Prothrombin time of patient / Prothrombin time normal range)ISI

% Reticulocytes = no. reticulocyte in square A (large square) x 100 / np. RBCs in square B (small square) x 9

Transferrin saturation Transferrin saturation (% sat) = serum iron (ug/dL) x 100 / TIBC (ug/dL)

Absolute Reticulocyte Count ARC = [reticulocytes (%)/100] RBC count (x 1012/L)

SUMMARY OF REFERENCE RANGES

Corrected Reticulocyte Count Corrected Reticulocyte Count (%) = reticulocytes (%) x patient’s HCT (%)/45 Reticulocyte Production Index Reticulocyte Production Index = reticulocyte (%) x (HCT (%) / 45) / maturation time Reticulocyte Production Index = corrected reticulocyte count / maturation time Rule of Three RBC x 3 should equal roughly Hgb Hgb x 3 should equal roughly Hct Red Cell Distribution Width RDW (%) = Standard deviation (SD) of MCV/ Mean MCV x MCV

Hemoglobin Newborn = 15-20 g/dL Female = 12-16 g/dL Male = 13-18 g/dL Hematocrit At birth = 45-60% Female = 36-48 % Male = 40-55% Red Blood Cell (RBC) Count Newborn = 5-6.5 x 1012 /L Female = 3.6-5.6 x 1012 /L Male = 4.2-6 x 1012 /L White Blood Cell (WBC) Count Newborn = 10-30 x 109 /L

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Notre Dame of Marbel University Medical Technology Department Medical Laboratory Science Study Notes in CLINICAL HEMATOLOGY A.M.J. Tamdang, A.M. Tomaro, V.C. Villapa, S.Y Yabes ( December 22, 2021)

1 year of Age = 6-17 x 109 /L Adult = 4-11 x 109 /L Differential Count (WBC) Neutrophil = 1.5-7.4 x 109 /L (35-71%) Lymphocyte = 1.4.4 x 109 /L (24-44%) Monocyte = 0.1-1.0 x 109 /L (1-10%) Band Neutrophil = 0.0-0.7 x 109 /L (0-6%) Eosinophil = 0.0-0.4 x 109 /L (0-4%) Basophil = 0.0-0.2 x 109 /L (0-2%) Mean Cell Volume (MCV) Normocytic = 80-100 Fl Mean Cell Hemoglobin Concentration Normochromic = 32-36 g/dL Mean Cell Hemoglobin Normal = 28-32 pg Red Cell Distribution Width Normal = 11.5-14.5% Platelet Count Normal = 150-450 x 109 /L Reticulocyte Count Normal = 0.5-1.5% Erythrocyte Sedimentation Rate (ESR) Westergren Method Children = 0-10 mm Female = 0-15 mm Male = 0.10 mm Wintrobe Method Female = 0-20 mm/hr Male = 0-9 mm/hr Bleeding Time Modified Duke Method = 70% Other Tests done in Hematology Section Fibrinogen = 1.5 - 4.5 g/L D-Dimers (NOT DVT/PE cut off value) = 0.1 - 0.45 mg/L FEU Antithromin III = 80 - 120 iu/dL Protein S = 60 – 140 iu/dL Protein C = 70 – 130 iu/dL Thrombin Time = 13-20 iu/dL Lupus Anticoagulant =...