Kami Export - Siddharth Bandari - DNA Replication - Labeling PDF

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Answer key for a biology assignment. It is about DNA....

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Sid Bandari Name:____________________________________________ Date:_________

DNA Replication Primase

Ligase

3'

5'

Okazaki fragment Lagging strand

Polymerase Leading strand

Single-Strand binding proteins

Helicase

Identify the structure Helicase

1. _____________________________ Enzyme that unwinds DNA

Okazaki fragments

2. _____________________________ Fragments of copied DNA created on the lagging strand

leading strand 3. _____________________________ The strand that is copied in a continuous way, from the 3’ to 5’ direction

ligase 4. _____________________________ Binds Okazaki fragments polymerase 5. _____________________________ Builds a new DNA strand by adding complementary bases helicase 6. _____________________________ Stabilizes the DNA molecule during replication leading 7. _____________________________ Strand that is copied discontinuously because it is traveling away from helicase

Primase 8. _____________________________ Initiates the synthesis DNA by creating a short RNA segment at replication fork

9. Place the events in the correct order: 2 DNA polymerase adds nucleotides in the 5’ to 3’ direction ____

4 Replication fork is formed ____ 3 DNA polymerase attaches to the primer ____

1 Okazaki fragments are bound together by ligase ____ 5 DNA helicase unwinds DNA ____ one strand from each parent is conserved and remains intact after DNA 10. Why is replication called “semi-conservative?” ___________________________________________________ replication.

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Name:____________________________________________ Date:_________

DNA Replication - Labeling

(with word bank)

DNA polymerase 3’ 5’ Single Strand Binding Proteins

DNA Ligase Helicase

Okazaki fragment Leading Strand

DNA Primase Lagging Strand

Identify the structure 1. _____________________________ Enzyme that unwinds DNA 2. _____________________________ Fragments of copied DNA created on the lagging strand 3. _____________________________ The strand that is copied in a continuous way, from the 3’ to 5’ direction 4. _____________________________ Binds Okazaki fragments 5. _____________________________ Builds a new DNA strand by adding complementary bases 6. _____________________________ Stabilizes the DNA molecule during replication 7. _____________________________ Strand that is copied discontinuously because it is traveling away from helicase 8. _____________________________Initiates the synthesis DNA by creating a short RNA segment at replication fork 9. Place the events in the correct order: ____ DNA polymerase adds nucleotides in the 5’ to 3’ direction ____ Replication fork is formed ____ DNA polymerase attaches to the primer ____ Okazaki fragments are bound together by ligase ____ DNA helicase unwinds DNA

10. Why is replication called “semi-conservative?” ___________________________________________________

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