Lab 1A - chem 108 PDF

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chem 108...

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Lam ! Han Lam ID: 16046516 Professor: Dr. Dontarie Stalling TA: Xandra Nuqui Chem 108 5 October 2020 Lab 1A: Laboratory IntroductionPipetting practice and the Spec20 spectrophotometer Key Principles 1. To prepared sample #22 we used the equation of dilution (M1V1=M2V2), !" !" (14.58#$)*(3 mL) = (2500 )*(x= amount of stock solution needed) #$ x= 17.4960 uL Adding H20 to the 17.4960 uL of 0.25% bromophenol blue to make thr total value is 3.0 mL.

1

Lam

2

! 2.

Tube

0.25% (w/v) bromophenol bromophenol blue (uL) blue (ug/mL)

H2O to A591 make 3 mL (Test (uL) Tube 1)

A591 (Test Tube 2)

A591 A591 A591 (Test (Test (Zero Tube 3) Tube avg) Adj avg)

1, 2, 3

0

0.000

3000.00

0.060

0.062

0.061

0.061

0

4, 5, 6

2.08

2.496

2997.50

0.189

0.196

0.193

0.193

0.132

7, 8, 9

4.17

5.004

2995.00

0.331

0.344

0.337

0.337

0.276

10, 11 12

6.25

7.500

2992.50

0.468

0.487

0.477

0.477

0.416

13, 14, 15

8.33

9.996

2990.00

0.583

0.606

0.594

0.594

0.533

16, 17, 18

10.42

12.504

2987.50

0.827

0.860

0.843

0.843

0.782

19. 20, 21

12.5

15.000

2985.00

0.903

0.939

0.921

0.921

0.860

22, 23, 24

14.58

17.496

2982.50

1.029

1.070

1.049

1.049

0.988

25, 26, 27

16.67

20.004

2980.00

1.170

1.217

1.193

1.193

1.132

28, 29, 30

18.75

22.500

2977.50

1.326

1.379

1.352

1.352

1.291

31, 32, 33

20.83 !

24.996

2975.00

1.461

1.519

1.490

1.490

1.429

Lam ! 3. Linear Regression Equation: Y = 0.069*X - 0.00646 m = 0.069 b = -0.00646

3...