Lab Report 5 PDF

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L3 Lab Report 5: Nematodes and Fecal Testing Cont. Fecal Testing Continued 1. Each student will run each of the following Fecal Flotation Procedures: a. Fecalyzer Animal Signalment: Roxy, Canine, Mastiff, DOB: 4/24/18, owner: Jane Stewart Gross Exam: Normal color, no visible parasites, no blood, no mucous, no foreign material, normal consistency score= 4 Steps for test: 1. Lift cap but leave the green insert in the clear tube. Insert the stool sample into the small end of the insert. 2. Fill the green insert with a flotation solution half way. Rotate the green insert in the clear tube but twisting back and forth, allowing the ova to separate from the fecal sample. 3. Push the green insert firmly in place with a tongue depressor or thumbs. Fill the rest of the insert and form a meniscus. 4. Place a cover slip on the meniscus for 15-20 minutes. Apply cover slip to a fresh slide. 5. Examine the slide under 10x. Result: Negative b. Ovatector Animal Signalment: Sybil, Canine, Unknown breed, DOB: unknown, sample collected 9/20/18, owner: Lindsey Marthy, #2302983850 Gross Exam: Normal color, no visible parasites, no blood, no mucous, no foreign material, normal consistency score= 5 Steps for test: 1. Remove spatula from device and collect fecal sample into the center receptacle. Return the clear tube atop the center receptacle. 2. Fill tube half way with a flotation solution. Mix fecal sample with flotation solution and push down gently with the strainer. 3. Fill the rest of the tube with the flotation solution and form a meniscus. 4. Place a cover slip over the meniscus and let sit for 15 minutes, to allow the ova to float through the strainer up to the slip. 5. Apply cover slip to a slide and examine under 10x. Results: Negative

Nematode Slides: Examine all slides below and sketch your findings. 1. Ancylostoma caninum adult (female)  Common name: Hookworm

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https://en.wikipedia.org/wiki/Ancylostoma_caninum

2. Necator americanus ova  Common name: human hookworm- similar to SA hookworm

https://helminthictherapywiki.org/wiki/index.php/Gallery

3. Trichuris Female Adult  Common name: Whipworm

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rm-

trichuris-trichiura/

4. Trichuris ova  Common name: Whipworm Height: 30 microns – Length: 50 microns

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html

5. Ascaris ova  large animal roundworm Height: 50 microns – Length: 60 microns

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https://en.wikipedia.org/wiki/Ascaris_lumbricoides

6. Toxocara canis ova  Canine Roundworm

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https://web.stanford.edu/group/parasites/ParaSites2005/Toxocariasis/whole%20thing.htm

Genus name: Tocascaris leonine – Common name: Roundworm

https://en.wikipedia.org/wiki/Toxascaris_leonina Genus name: Baylisascaris procyoncis – Common name: Raccoon roundworm

https://www.cdc.gov/dpdx/baylisascariasis/index.html 7. Acanthocephala adult  Common name: Thorny headed worms

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http://www2.biology.ualberta.ca/parasites/ParPub/text/text/acant01b.htm

8. Acanthocephalan ova  Common name: Thorny headed worm

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7925344

9. Haemonchus contortus  Common name: Barber pole worm

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http://www.wormboss.com.au/worms/roundworms/barbers-pole-worm.php

10. Oesophagostomum radiatum  Common name: Nodular worm

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http://parasitesworld.com/oesophagostomum-radiatum-3/ 11. Trichinella in muscle tissue  Common name: The Pork worm

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 https://www.cdc.gov/dpdx/trichinellosis/index.html

3.Scan the Strongyloides wet mount. Draw and include image.

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http://www.phsource.us/PH/HELM/PH_Parasites/Strongyloidiasis.htm  4. Baermann technique. a. What is the Baermann technique most commonly used for? The Baermann technique is most commonly used for lungworm in felines and threadworm in canines. https://www.plantpath.iastate.edu/tylkalab/content/extractingnematodes-soil-baermann-funnel ...