Lab2-Hand-Hygiene-Lab-Results(2) PDF

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Microbiology Lab Safety EXPERIMENT 1: IMPORTANCE OF HAND HYGIENE Data Tables Record growth as: (0) = no growth; (+) = little growth (less than or equal to 5 colonies); (++) = moderate growth (6 – 20 colonies);(++ +) = heavy growth (20 – 50 colonies); (++++) = confluent growth (no individual colonies; rather, they grow as a “lawn” and are uncountable). What is a colony? A colony is a result of 1 bacterial cell (or yeast cell) dividing over and over again in an exponential fashion as described/shown in lecture video chapter 2. Example of colony growth: Your colonies may look a little different in size and color depending of types of species that grow.

Experiment 1 Colony Growth Plate #1 #2

Condition Hand without Washing Hand with Washing

Growth Day 1

Growth Day 2

Growth Day 3

Growth Day 4

Growth Day 5

0

+

++

++

+++

0

+

++

+++

++++

#3

Yeast

0

0

+

+

+

#4

Deionized Water

0

0

0

+

+

Take a picture of plate #3 (yeast plate) showing growth on day 5. Include your ID in the photo. Photo: (I apologize for the small bubble that were formed when I made the petri dish for #3 plate, other plates were much better)

©eScience Labs, 2018

Microbiology Lab Safety

©eScience Labs, 2018

Microbiology Lab Safety Post-Lab Questions 1. What purpose did plating the yeast serve in this experiment?

Planting the yeast serve as reference to compare the growth of what a microorganism would look like on an agar plate when they grow.

2. What purpose did the water serve?

The deionized water serves as a control plate for the experiment where we could base no growth. 3. Which of the two hand treatments grew the most colonies?

In my experiment, the hand with washing treatment ended up growing the most colonies at the end. It could be possibly from the paper towel I used which was in my kitchen and may have been contaminated.

4. What effect did handwashing have on the presence of bacteria?

Hand washing kills the bacteria that are accumulated at the high points of the skin. 5. Were there any colonies present on plate #2? What could account for the presence of these colonies?

Yes, there were colonies present on plate #2. The paper towel that I used was sitting in the kitchen and they might have exposed to something, which cause the growth.

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