Midterm Myco Viro Topic 3- Introduction to Mycology PDF

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Mycology and VirologyMidterm Topic 3RMT2022Microbiology;;[TRANS] TOPIC 3: INTRODUCTION TO MYCOLOGY WITH LABORATORY DIAGNOSIS**OUTLINE INTRODUCTION GENERAL CHARACTERISTICS OF FUNGI A.** Gram Staining B. Eukaryotic organisms C. Motility D. Cell Wall E. Growth Requirements F. Growth Phase G. Basic Stru...

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Mycology and Virology

;;

RMT 2022

Microbiology Midterm Topic 3

[TRANS] TOPIC 3: INTRODUCTION TO MYCOLOGY WITH LABORATORY DIAGNOSIS Cell Wall Components

OUTLINE 1) 2)

3) 4) 5)

INTRODUCTION GENERAL CHARACTERISTICS OF FUNGI A. Gram Staining B. Eukaryotic organisms C. Motility D. Cell Wall E. Growth Requirements F. Growth Phase G. Basic Structure H. Type of Fungi Based on Reproduction

Cell Membrane Composition

Specimen Collection, Handling, & Processing Laboratory Identification Methods

INTRODUCTION MYCOLOGY is the study of the eukaryotic fungi, including yeasts, moulds, and mushroom.  MEDICAL MYCOLOGY is the study of fungi that produce pathology or disease in humans and animals including their ecology and epidemiology  DIAGNOSTIC MYCOLOGY is the study of the general characteristics, pathology & pathogenesis, and laboratory tests & methods of medically important fungi. that aids in their identification and treatment GENERAL CHARACTERISTICS OF FUNGI  Fungi constitute an extremely diverse group of organisms and are generally classified as molds or yeasts.  Some have been recognized as classic pathogens, whereas others are recognized only as environmental saprobes, living on nonliving material GRAM STAINING REACTION  Fungi stains gram positive EUKARYOTIC ORGANISMS  Have a true nucleus bounded by a nuclear membrane. Also have endoplasmic reticulum for protein synthesis & mitochondria for energy production o Fungi are Eukaryotic, classified in their own separate kingdom, the Kingdom Fungi o Lack chlorophyll, thus heterotrophic MOTILITY  Non motile CELL WALL  Definite cell wall that contains complex carbohydrates such as chitin, mannan, glucan, & chitosan Ergosterol present in the cell membrane  NOTE! o Grow best at neutral pH o Lack susceptibility to antibacterial agents Table 1 – Fungi Vs. Bacteria Fungi Bacteria Eukaryotic “has Prokaryotic “no Cell Structure 

nucleus”

nucleus

Cytoplasmic Components Size Spores basic reproductive unit of fungi Mode of Reproduction

Polysaccharide:  Mannan  Glucan  Chitin  Other polysaccharides Sterol (Ergosterol) – components of membrane; site of attacks of antifungal; not selective it will attack the cells and hormones such as testosterone and progesterone causing impotene endoplasmic reticulum (rough), mitochondria >5um for reproduction

Peptidoglycan/ Muramic acid

Sexual rep. Asexual rep

Binary fission

No sterol

no ER, mitochondria...