Module 9 problem-solving sheet PDF

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Practice sheet on DNA replication and Gene mutation causes...

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Problem-solving Worksheet for Module 9 SP2021 1. Below shows the start of replication on a stretch of DNA.  For each color, state whether it is the leading strand or the lagging strand. Blue: lagging strand Red: leading strand Purple: leading strand Green: lagging strand

2.Here is a picture of a replication fork: For A,B, C, and D: label the ends as either 5’ or 3’ For E-J, label using these terms: leading strand, lagging strand, Okazaki fragments, replication fork, and DNA polymerase. A= 3’ B= 5’ C= 3’ D= 5’ F = lagging strand G = leading strand H= Okazaki fragment I= DNA polymerase J = replication fork

3. Below is a short sequence of DNA. a. Shown here is a template strand of DNA. Write the sequence of the complementary DNA underneath this strand and designate the 5’ and 3’ ends)

5’ G T A C A G T G A 3’ 3’ C

A T

G

T

C

A

Use this diagram to answer questions 4-6

C

T 5’

4. Which strand is the lagging strand: the one marked 1 or the one marked 2? The one marked 2 5. The enzyme marked 3 is adding the nucleotides. What is the name of this enzyme? DNA Polymerase III 6. The enzyme marked 4 is breaking the hydrogen bonds between the bases. What is the name of this enzyme? Helicase 7. Which of the following are true (check ALL that are correct)?  DNA polymerase can add nucleotides to _____. a.) a free 3' OH end of a growing DNA chain b.) a free 5' OH end of a growing DNA chain c.) a free 5' phosphate end of a growing DNA chain d.) a free 3' OH end of an RNA primer e.) a free 5' OH end of an RNA primer f.) a free 5' phosphate end of a growing DNA chain

8. In the diagram above, which DNA polymerase III is making the leading strand: the one on the top or the one on the bottom? The one on the top 9. What is the function of the single stranded binding proteins? To hold the DNA strands apart during replication

10. The enzyme that replaces the nucleotides of the RNA primer with the appropriate DNA nucleotides is not in this diagram. What is it called? DNA polymerase I 11. Which of the following describes the function of RNA primase during DNA replication? a.) b.) c.) d.)

To unwind the double helix to prime it for replication To prime DNA for replication by preventing the formation of knots To add a DNA primer to enable replication of DNA To add a RNA primer to enable replication of DNA...