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Official Methods of Analysis 20th Edition (2016) Front Matter Standard Method Performance Requirements (SMPRs®): AOAC SMPR® 2011.009 Standard Method Performance Requirements for Cr, Mo, and Se in Infant Formula and Adult/Pediatric Nutritional Formula Revised January 2015 to correct Table 1, footnote...

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Official Methods of Analysis 20th Edition (2016) Front Matter

Standard Method Performance Requirements (SMPRs®): AOAC SMPR® 2011.009 Standard Method Performance Requirements for Cr, Mo, and Se in Infant Formula and Adult/Pediatric Nutritional Formula Revised January 2015 to correct Table 1, footnote b, to read: “µg/kg reconstituted final product” AOAC SMPR® 2011.011 Standard Method Performance Requirements for in vitro Determination of Total Antioxidant Activity in Foods, Beverages, Food Ingredients, and Dietary Supplements Approved by AOAC Stakeholder Panel on Strategic Foods Analytical Method (SPSFAM) and revised March 2013 to not require establishing equivalency of results NEW AOAC SMPR® 2012.001 Standard Method Performance Requirements for Flavanols in Foods and Beverages NEW AOAC SMPR® 2012.002 Standard Method Performance Requirements for Whey Protein:Casein Ratio in Infant Formula NEW AOAC SMPR® 2012.003 Standard Method Performance Requirements for Vitamin A in Preblends, Premixes, and Pure Materials NEW AOAC SMPR® 2012.004 Standard Method Performance Requirements for Vitamin D in Preblends, Premixes, and Pure Materials NEW AOAC SMPR® 2012.005 Standard Method Performance Requirements for Vitamin E in Preblends, Premixes, and Pure Materials NEW AOAC SMPR® 2012.006 Standard Method Performance Requirements for Vitamin K in Preblends, Premixes, and Pure Materials NEW AOAC SMPR® 2012.007 Standard Method Performance Requirements for Heavy Metals in a Variety of Foods and Beverages NEW AOAC SMPR® 2012.008 Standard Method Performance Requirements for Iodine in Infant Formula and Adult/Pediatric Nutritional Formula NEW AOAC SMPR® 2012.009 Standard Method Performance Requirements for Pantothenic Acid in Infant Formula and Adult/Pediatric Nutritional Formula NEW AOAC SMPR® 2012.010 Standard Method Performance Requirements for L-Carnitine in Infant Formula and Adult/Pediatric Nutritional Formula NEW AOAC SMPR® 2012.011 Standard Method Performance Requirements for Fatty Acids, Including LCPUFAs, in Infant Formula and Adult/Pediatric Nutritional Formula NEW AOAC SMPR® 2012.012 Standard Method Performance Requirements for Vitamin C in Infant Formula and Adult/Pediatric Nutritional Formula NEW AOAC SMPR® 2012.013 Standard Method Performance Requirements for Total Choline in Infant Formula and Adult Pediatric Nutritional Formula NEW AOAC SMPR® 2013.001 Standard Method Performance Requirements for Hypericins, Hyperforins, and Flavonoids in St. John’s Wort (Hypericum perforatum) and Other Hypericum spp. NEW AOAC SMPR® 2014.001 Standard Method Performance Requirements for Vitamin K in Infant Formula and Adult/Pediatric Nutritional Formula NEW AOAC SMPR® 2014.002 Standard Method Performance Requirements for Fructans in Infant Formula and Adult/Pediatric Nutritional Formula NEW AOAC SMPR® 2014.003 Standard Method Performance Requirements for Galactooligosaccharides (GOS) in Infant Formula and Adult/Pediatric Nutritional Formula NEW AOAC SMPR® 2014.004 Standard Method Performance Requirements for Minerals and Trace Elements in Infant Formula and Adult/Pediatric Nutritional Formula

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If you have any questions of a technical nature or suggestions for editorial changes, please e-mail us at [email protected]. © 2016 AOAC INTERNATIONAL

NEW AOAC SMPR® 2014.005 Standard Method Performance Requirements for Biotin in Infant Formula and Adult/Pediatric Nutritional Formula NEW AOAC SMPR® 2014.006 Standard Method Performance Requirements for Detection and Identification of Variola Virus in Aerosol Collection Filters and/or Liquids NEW AOAC SMPR® 2014.007 Standard Method Performance Requirements for Authentication of Selected Vaccinium species (Anthocyanins) in Dietary Ingredients and Dietary Supplements NEW AOAC SMPR® 2014.008 Standard Method Performance Requirements for Screening Method for Selected Adulterants in Dietary Ingredients and Supplements Containing Chondroitin Sulfate NEW AOAC SMPR® 2014.009 Standard Method Performance Requirements for Total Chondroitin Sulfate in Dietary Ingredients and Supplements NEW AOAC SMPR® 2014.010 Standard Method Performance Requirements for Identification of Phosphodiesterase Type 5 (PDE5) Inhibitors in Dietary Ingredients and Supplements NEW AOAC SMPR® 2014.011 Standard Method Performance Requirements for Quantitative Methods for Determination of Phosphodiesterase Type 5 (PDE5) Inhibitors in Dietary Ingredients and Supplements NEW AOAC SMPR® 2014.012 Standard Method Performance Requirements for Screening Methods for Phosphodiesterase Type 5 (PDE5) Inhibitors in Dietary Ingredients and Supplements NEW AOAC SMPR® 2014.013 Standard Method Performance Requirements for Amino Acids in Infant and Adult/Pediatric Nutritional Formula NEW AOAC SMPR® 2014.014 Standard Method Performance Requirements for Selected Carotenoids in Infant and Adult/ Pediatric Nutritional Formula NEW AOAC SMPR® 2014.015 Standard Method Performance Requirements for Chloride in Infant and Adult/Pediatric Nutritional Formula NEW AOAC SMPR® 2014.016 Standard Method Performance Requirements for Fluoride in Infant and Adult/Pediatric Nutritional Formula NEW AOAC SMPR® 2014.017 Standard Method Performance Requirements for Detection of Salmonella species in Romaine Lettuce and Baby Spinach NEW AOAC SMPR® 2015.001 Standard Method Performance Requirements for Sodium Fluoroacetate (“Compound 1080”) in Infant Formula NEW AOAC SMPR® 2015.002 Standard Method Performance Requirements for Total Vitamin B1 (Thiamine) in Infant Formula and Adult/Pediatric Nutritional Formula NEW AOAC SMPR® 2015.003 Standard Method Performance Requirements for Total Vitamin B2 (Riboflavin) in Infant Formula and Adult/Pediatric Nutritional Formula NEW AOAC SMPR® 2015.004 Standard Method Performance Requirements for Total Vitamin B3 (Niacin) in Infant Formula and Adult/Pediatric Nutritional Formula NEW AOAC SMPR® 2015.005 Standard Method Performance Requirements for Total Vitamin B6 (Pyridoxine) in Infant Formula and Adult/Pediatric Nutritional Formula NEW AOAC SMPR® 2015.006 Standard Method Performance Requirements for Quantitation of Arsenic Species in Selected Foods and Beverages NEW AOAC SMPR® 2015.007 Standard Method Performance Requirements for Withanolide Glycosides and Aglycones of Ashwagandha (Withania somnifera) NEW AOAC SMPR® 2015.008 Standard Method Performance Requirements for Alkaloids of Mitragyna speciosa NEW AOAC SMPR® 2015.009 Standard Method Performance Requirements for Estimation of Total Phenolic Content Using the Folin-C Assay NEW AOAC SMPR® 2015.010 Standard Method Performance Requirements for Identification of Selected Cinnamomum spp. Bark in Dietary Supplement Raw Materials and/or Finished Products NEW AOAC SMPR® 2015.011 Standard Method Performance Requirements for Detection of Coxiella burnetii NEW AOAC SMPR® 2015.012 Standard Method Performance Requirements for Identification of Venezuelan Equine Encephalitis Virus NEW AOAC SMPR® 2015.013 Standard Method Performance Requirements for Detection of Staphylococcal enterotoxin B NEW AOAC SMPR® 2015.014 Standard Method Performance Requirements for Determination of Catechins, Methyl Xanthines, Theaflavins, and Theanines in Tea Dietary Ingredients and Supplements

© 2016 AOAC INTERNATIONAL

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NEW AOAC SMPR® 2015.015 Standard Method Performance Requirements for Aloin A and Aloin B in Dietary Supplement Products and Ingredients NEW AOAC SMPR® 2015.016 Standard Method Performance Requirements for Vitamin D in Dietary Finished Products and Ingredients Chapter 2

942.02 (2.6.29) Zinc in Fertilizers Included full text 942.03 (2.6.30) Zinc in Fertilizers Included full text

Chapter 4

971.09 (4.4.04) Pepsin Digestibility of Animal Protein Feeds Updated October 2012: B(a): Deleted “Agitator and bottles available from Terry Sims (1901 Laurel Rd, Oceanside, CA 92054, USA, Tel: +1-760-722-4119).” NEW 2014.10 (4.7.07) Dietary Starch in Animal Feeds and Pet Food NEW 2012.25 (4.10.07) Residues of Three Triphenylmethane Dyes and Their Metabolites (Malachite Green, Leucomalachite Green, Crystal Violet, Leucocrystal Violet, and Brilliant Green) in Aquaculture Product Final Action 2016: Approved by the Official Methods Board in February 2016

Chapter 5

2008.01 (5.2.07) Lasalocid Sodium in Animal Feeds and Premixes Final Action 2013 2008.08 (5.3.25) Decoquinate in Animal Feeds Final Action 2013 2008.09 (5.3.26) Oxytetracycline/Oxytetracycline Hydrochloride in Animal Feed, Fish Feed, and Animal Remedies Final Action 2013

Chapter 6

955.14 (6.2.01) Testing Disinfectants Against Salmonella enterica Approved by Methods Committee on Antimicrobial Efficacy Testing and revised March 2013 to include editorial and procedural revisions 955.15 (6.2.04) Testing Disinfectants Against Staphylococcus aureus Approved by Methods Committee on Antimicrobial Efficacy Testing and revised March 2013 to include editorial and procedural revisions Revised September 2013 to include editorial and procedural revisions: C(f): Deleted “Maximum dilution of germicide which kills test organism on 10 carriers in 10 min interval represents presumed maximum safe use dilution for practical disinfection.” and “Note: While killing in 10 of 10 replicates specified provides reasonably reliable index in most cases, killing in 59 of 60 replicates is necessary for confidence level of 95%.” Included new section C(h) on Performance Standard and Note 964.02 (6.2.06) Testing Disinfectants Against Pseudomonas aeruginosa Approved by Methods Committee on Antimicrobial Efficacy Testing and revised March 2013 to include editorial and procedural revisions Revised September 2013 to include editorial and procedural revisions: C(f): Deleted “Maximum dilution of germicide which kills test organism on 10 carriers in 10 min interval represents presumed maximum safe use dilution for practical disinfection.” and “Note: While killing in 10 of 10 replicates specified provides reasonably reliable index in most cases, killing in 59 of 60 replicates is necessary for confidence level of 95%.” Included new section C(h) on Performance Standard and Note 960.09 (6.3.03) Germicidal and Detergent Sanitizing Action of Disinfectants Approved by Methods Committee on Antimicrobial Efficacy Testing and revised March 2013 to include editorial and procedural revisions 961.02 (6.3.04) Germicidal Spray Products as Disinfectants Approved by Methods Committee on Antimicrobial Efficacy Testing and revised March 2013 to include editorial and procedural revisions 966.04 (6.3.05) Sporicidal Activity of Disinfectants Approved by Methods Committee on Antimicrobial Efficacy Testing and revised March 2013 to include editorial and procedural revisions v

© 2016 AOAC INTERNATIONAL

965.12 (6.3.06) Tuberculocidal Activity of Disinfectants Approved by Methods Committee on Antimicrobial Efficacy Testing and revised December 2012 to include editorial and procedural revisions 2008.05 (6.3.09) Efficacy of Liquid Sporicides Against Spores of Bacillus subtilis on Hard Nonporous and Porous Surfaces Approved by Methods Committee on Antimicrobial Efficacy Testing and revised March 2013 to include editorial and procedural revisions Chapter 7

2011.26 (7.8.12) Phenols and Phenates in Disinfectant Formulations Final Action 2013 Moved from Chapter 6 (6.1.04) to Chapter 7 (7.8.12) Revised December 2012 to correct conversion factors in Table 2011.26E

Chapter 9

NEW 2013.06 (9.1.10) Arsenic, Cadmium, Mercury, and Lead in Foods

Chapter 10

2007.01 (10.1.04) Pesticide Residues in Foods by Acetonitrile Extraction and Partitioning with Magnesium Sulfate

NEW 2015.01 (9.2.42) Heavy Metals in Food

Final Action 2013 2010.03 (10.6.32) Bifenthrin in Technical and Selected Formulated Products Final Action 2013 2007.08 (10.7.05) Low-Level Pesticide Residues in Soft Drinks and Sports Drinks Final Action 2013 2007.09 (10.7.06) Pesticide Residues (>0.5 μg/L) in Soft Drinks and Sports Drinks Final Action 2013 Chapter 11

973.51 (11.1.14) Chloride in Water Revised January 2015 to read: B(a) ... “Dissolve 824.1 mg NaCl, previously dried at 140°C for 2 h and cooled in a desiccator, in Cl-free H2O and dilute to 1 L.” ...

Chapter 16

972.40 (16.14.23) Light Filth in Pepper Revised January 2015 to correct cross reference for sieve

Chapter 17

NEW 2014.05 (17.2.11) Enumeration of Yeast and Mold in Food NEW 2015.13 (17.2.12) Enumeration of Aerobic Bacteria in Food 996.10 (17.4.01B) Transia® AG EHEC for the Detection of Escherichia coli O157:H7 in Selected Foods Approved by Methods Committee on Microbiology and revised December 2015 to indicate new test kit name and additional minor editorial revisions 2005.04 (17.4.01E) Escherichia coli O157:H7 in Selected Foods Approved by Methods Committee on Microbiology and revised December 2012 to add an increased test portion size (375 g) 2005.05 (17.4.06A) Shigatoxin genes, from E. coli O157:H7, in Selected Foods Approved by Methods Committee on Microbiology and revised December 2012 to add an increased test portion size (375 g) 2000.14 (17.4.07) Reveal for E. coli O157:H7 Test in Selected Foods and Environmental Swabs Table 2000.14B: Corrected table numbering 999.08 (17.9.25) Transia® AG Salmonella EIA for the Visual or Instrumental Detection of Motile and Nonmotile Salmonella in All Foods Approved by Methods Committee on Microbiology and revised December 2015 to indicate new test kit name and additional minor editorial revisions 2009.03 (17.9.35) Salmonella in Foods and Environmental Surfaces Revised First Action 2015: Approved by ERP in March 2015 for matrix extension NEW 2013.01 (17.9.36) Salmonella in a Variety of Foods Final Action 2016: : Approved by the Official Methods Board in February 2016 NEW 2013.02 (17.9.37) Salmonella Species in a Variety of Foods and Environmental Surfaces Final Action 2016: : Approved by the Official Methods Board in February 2016

© 2016 AOAC INTERNATIONAL

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NEW 2013.09 (17.9.38) Salmonella in Selected Foods Revised First Action 2014 Final Action 2016: : Approved by the Official Methods Board in February 2016 NEW 2013.14 (17.9.39) Identification of Salmonella spp. from Colony Picks Final Action 2016: : Approved by the Official Methods Board in February 2016 NEW 2014.01 (17.9.40) Salmonella in Selected Foods 996.14 (17.10.07) Transia® AG Listeria for the Detection of Listeria monocytogenes and Related Listeria Species in Selected Foods and Environmental Surfaces Approved by Methods Committee on Microbiology and revised December 2015 to indicate new test kit name and additional minor editorial revisions NEW 2013.10 (17.10.14) Listeria Species in a Variety of Foods and Environmental Surfaces Final Action 2016: : Approved by the Official Methods Board in February 2016 NEW 2013.11 (17.10.15) Listeria monocytogenes in a Variety of Foods Final Action 2016: : Approved by the Official Methods Board in February 2016 NEW 2014.06 (17.10.16) Listeria species in Selected Foods and Environmental Surfaces NEW 2014.07 (17.10.17) Listeria monocytogenes in Selected Foods and Environmental Surfaces NEW 2012.06 (17.14.06) Bacillus anthracis in Aerosol Collection Samples 2012.02 (17.15.02) Gram-Positive Bacteria Identification Included reference: “J. AOAC Int. 95, 1425(2012)” Final Action 2016: : Approved by the Official Methods Board in February 2016 NEW 2013.08 (17.16.01) Ricin Powder on Nonporous Surfaces Chapter 23

2011.23 (23.1.19) Determination and Confirmation of Parent and Total Ractopamine in Bovine, Swine, and Turkey Tissues Final Action 2012: Revised to reflect Final Action status and included reference to Final Action paper [J. AOAC Int. 96, 917(2013)] 2011.24 (23.1.20) Determination and Confirmation of Narasin and Monensin in Chicken, Swine and Bovine Tissues Final Action 2012: Revised to reflect Final Action status and included reference to Final Action paper [J. AOAC Int. 96, 902(2013)] NEW 2013.07 (23.1.21) Determination and Confirmation of Nicarbazin in Chicken Tissues

Chapter 28

NEW 2013.12 (28.1.49) Total Carbohydrates in Wine and Wine-Like Beverages

Chapter 30

NEW 2014.09 (30.1.35) High-Throughput Analytical Techniques for Determination and Confirmation of Residues of 653 Multi-Class Pesticides and Chemical Pollutants in Tea

Chapter 31

NEW 2012.24 (31.5.14) Flavanol and Procyanidin (by Degree of Polymerization 1-10) Content of Chocolate, Cocoa Liquors, Powder(s), and Cocoa Flavanol Extracts NEW 2013.03 (31.5.15) Analysis of Cocoa Flavanols and Procyanidins (DP 1-10) in Cocoa-Containing Ingredients and Products NEW 2013.04 (31.5.16) Catechin and Epicatechin Enantiomers in Cocoa-Based Ingredients and Products

Chapter 32

NEW 2014.03 (32.1.45) Gluten in Rice Flour and Rice-Based Food Products

Chapter 33

2006.06 (33.2.67) Lactose in Milk

NEW 2015.05 (32.2.11) Partially Hydrolyzed Gluten in Fermented Cereal-Based Products Final Action 2013 920.119 (33.6.11) Color Additives in Butterfat General Test section: Revised July 2014 to reference Chapter 46 on Color Additives, not Chapter 34 Chapter 35

977.13 (35.1.32) Histamine in Seafood Revised November 2012: C(b): 79.33 mL (not 73.33 mL) 85% (15 M H3PO4) NEW 2014.08 (35.1.46) Polycyclic Aromatic Hydrocarbons (PAHs) in Seafood

Chapter 36

2006.05 (36.2.24) Site-Specific Deuterium/Hydrogen (D/H) Ratios in Vanillin Final Action 2013

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Chapter 39

972.22 (39.1.13) Phosphorus in Meat Include full text 970.42 (39.1.17) Nitrogen in Meat Include full text 2008.06 (39.1.39) Moisture and Fat in Meats Final Action 2013

Chapter 44

998.12 (44.4.18A) C-4 Plant Sugars in Honey Revised First Action 2013: Modification approved by Official Methods Board in January 2013 to add filtration and/or centrifugation Deleted parenthetical text below title: “(Method is used...concentration >7%)” Replaced statistical performance data with Table 998.12 A: Added the following sentence: “Addition of C-4 sugars (cane sugar/corn syrup) to honey increases the value of the stable carbon isotope ratio of the honey.” A: Replaced “is a measure” with “allows for the detection and an estimate of the amount” Protein, C: Replaced last sentence “Product is considered to contain significant C-4 sugars (primarily corn or cane) only at or above 7%.” to “Pure honey (free of corn or cane sugars), with an exception of a few unusual varieties, yields a value of C-4 sugars of ≤7%. Some unusual varieties may slightly exceed the range, but will have δ13C for honey which are in the normal range (more negative than –24.0‰).” Included reference to modification: “J. AOAC Int. 96, 607(2013)”

Chapter 45

997.08 (45.4.06A) Fructans in Food Products Revised April 2013: C(e): Included availability of Fructanase liquid 994.10 (45.4.10) Cholesterol in Foods Revised November 2014: C(b): Replaced “Hexamethyldisilane (HMDS).—CAS No. 1450-14-2” with “Hexamethyldisilazane (HMDS).—CAS No. 999-97-3”

Chapter 47

2012.03 (47.2.05) Antioxidant Capacity of Commercial Beverages Included reference: “J. AOAC Int. 95, 1558(2012)” 2012.04 (47.2.06) Antioxidant Activity in Foods and Beverages Included reference: “J. AOAC Int. 95, 1562(2012)” NEW 2012.23 (47.2.07) Total Antioxidant Activity

Chapter 49

2008.02 (49.2.32) Aflatoxins B1, B2, G1, and G2 and Ochratoxin A in Ginseng and Ginger Final Action 2013 NEW 2013.05 (49.3.08) Aflatoxins B1, B2, G1, and G2 in Olive Oil, Peanut Oil, and Sesame Oil 2011.02 (49.10.05) Paralytic Shellfish Toxins in Mussels, Clams, Oysters, and Scallops Final Action 2013

Chapter 50

2011.05 (50.1.28) Folate in Infant Formula and Adult/Pediatric Repealed 2014 2011.06 (50.1.29) Total Folates in Infant Formula and Adult Nutritionals Revised as follows: A: Six (not seven) folate vitamers are quantified and the total folates determined and expressed as folic acid D(a)(2): 4.8 mL (not 5 g) glac...