Pcb 2061 ch 11 - Chapter 11 Vocabulary Study Guide PDF

Preview

Summary

Chapter 11
Vocabulary
Study Guide...

Description

Which sigmas can be used to transcribe genes during some stress conditions? Sigma S, sigma 32

Listed below are sigma subdomains followed by its function. Which of the following pairings has an incorrect function assigned? H-T-H 1.1; blocks the exit pore of RNA

What is NOT the activity of the antibiotic rifampicin in fighting tuberculosis? Can be easily dislodged by other antibiotics.

What technique can be used to evaluate protein: DNA contacts on the single-stranded DNA? DNase I footprinting w/ Filter binding assay w/ GST pool down assays w/ DMS footprinting

What statement is FALSE? Some NIF genes have dual promoters, which can be turned on either by sigma 70 or sigma 54. Sigma 54 does not have REGION1.1. Sigma 54 activates the constitutive promoter of nitrogen starvation gene glnA. Sigma 54 is very unusual since it can bind promoter in the absence of RNAP core. Sigma 54 activates the constitutive promoter of nitrogen starvation gene glnA

In the transition from abortive cycling to elongation of transcription, which is TRUE?

Sigma looses affinity for the promoter DNA and RNAP core, and pressure of growing RNA chain dislodges sigma from the RNA exit pore.

Listed below are sigma subdomains followed by its function. Which of the following pairings has an incorrect function assigned? 4.2; melting of the -35 element

How does RNAP holoenzyme recognize different gene promoters? By binding alternative specialized sigma subunits, which recognize different cis-element sequences and various configurations of the promoter

Listed below are sigma subdomains followed by its function. Which of the following pairings has an incorrect function assigned? 2.3; contacts alpha CTD

How do sigma factors recognize promoter sequence? By sliding, hopping, or transfer between very AT-rich sequences located along DNA helix w/ By recognition of specific DNA cis-elements at position -10 and the equivalent of -35, as well as promoter configuration (distance between cis-elements)

What is the function of heparin in EMSA (gel retardation) and DNase I footprinting assays? Heparin is negatively charged; acts as a competitor to knock off any RNAP that is not in a stable open complex formation.

In the GST pull-down experiment, the entire bacterial core and distal promoter was used as a radioactive probe pre-bound with isolated GST-alpha CTD subunit. The outcome was a very sharp decline in the pulled-down radioactivity. What competitor was used to obtain this effect?

UP element...