Physio Ex Exercise 1 Activity 3 PDF

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PhysioEx Lab Report Exercise 1: Cell Transport Mechanisms and Permeability Activity 3: Simulating Osmotic Pressure Name: Kaley Faucher Date: 15 September 2020 Session ID: session-35980e73-df2c-8a50-9da0-f2c9a80d85c7

Pre-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1 Which of the following is true of osmosis? You correctly answered: It is a type of diffusion. 2 Which of the following occurs when a hypertonic solution is added to cells? You correctly answered: The cells shrink. 3 The variable that affects osmotic pressure is You correctly answered: the concentration of nondiffusing solutes. 4 The net movement of water would be into the cell in a You correctly answered: hypotonic solution.

Experiment Results Predict Questions 1 Predict Question 1: What effect do you think increasing the Na +Cl -concentration will have? Your answer: increased osmotic pressure. 2 Predict Question 2: What do you think will be the pressure result of the current experimental conditions? Your answer: pressure above the left beaker. Stop & Think Questions 1 Why do you think there is no pressure change? You correctly answered: Sodium is able to diffuse through the pores. 2 Why do you think there was no pressure change?

You correctly answered: There is no net movement of water because the solute concentrations are the same. Experiment Data Run Number

Solute

MWCO

Start Conc. L (mM)

Pressure L (mm Hg)

Start Conc. R (mM)

Pressure R (mm Hg)

Rate (mM/sec)

1

Na+ Cl-

20

5.00

170

0.00

0

0.0000

1

Albumin

20

0.00

170

0.00

0

0.0000

1

Glucose

20

0.00

170

0.00

0

0.0000

2

Na+ Cl-

20

10.00

340

0.00

0

0.0000

2

Albumin

20

0.00

340

0.00

0

0.0000

2

Glucose

20

0.00

340

0.00

0

0.0000

3

Na+

50

10.00

0

0.00

0

0.0167

Cl-

3

Albumin

50

0.00

0

0.00

0

0.0000

3

Glucose

50

0.00

0

0.00

0

0.0000

4

Na+ Cl-

100

8.00

0

0.00

0

0.0133

4

Albumin

100

0.00

0

0.00

0

0.0000

4

Glucose

100

0.00

0

0.00

0

0.0000

5

Na+ Cl-

100

0.00

0

0.00

0

0.0000

5

Albumin

100

0.00

0

0.00

0

0.0000

5

Glucose

100

8.00

0

8.00

0

0.0000

6

Na+ Cl-

200

0.00

0

0.00

0

0.0000

6

Albumin

200

0.00

0

0.00

0

0.0000

6

Glucose

200

8.00

0

0.00

0

0.0036

7

Na+

200

0.00

153

0.00

0

0.0000

Cl-

7

Albumin

200

9.00

153

0.00

0

0.0000

7

Glucose

200

0.00

153

8.00

0

0.0036

Post-lab Quiz Results You scored 50% by answering 2 out of 4 questions correctly. 1 If you double the concentration of a nondiffusible solute that is on one side of a membrane, the osmotic pressure will Your answer: be unchanged. Correct answer: double.

2 Which membrane did not allow Na + Cl - to pass through? You correctly answered: 20 MWCO. 3 When a solute is able to diffuse through a membrane Your answer: pressure changes occur. Correct answer: equilibrium is reached. 4 Water diffuses You correctly answered: toward solutes.

Review Sheet Results 1 Explain the effect that increasing the Na + Cl - concentration had on osmotic pressure and why it has this effect. How well did the results compare with your prediction? Your answer: There is an increased force of water to the high concentration side, resulting in increased osmotic pressure because of the high concentration of molecules on that side. 2 Describe one way in which osmosis is similar to simple diffusion and one way in which it is different. Your answer: Osmosis does not require energy, and neither does simple diffusion due to both moving molecules from an area of higher concentration to the area of lower concentration, they are both passive ways of diffusion. Osmosis differs from simple diffusion is that in simple diffusion the ions or membranes are transported across the membrane, and in osmosis, only water can be transported via a selectively permeable membrane. 3 Solutes are sometimes measured in milliosmoles. Explain the statement, “Water chases milliosmoles.” Your answer: Water will move by osmosis to from area with lower milliosmoles toward an area with higher milliosmoles concentration, meaning water chases the area that is higher in milliosmoles. 4 The conditions were 9 mM albumin in the left beaker and 10 mM glucose in the right beaker with the 200 MWCO membrane in place. Explain the results. How well did the results compare with your prediction? Your answer:

The albumin did not diffuse, because it is too large to fit through the filter, resulting in the left side having more pressure because minimal water molecules could diffuse....