PRAC 1 ID Biohazard v1 - BMS 533 PDF

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GROUP : AS201 TEAM :Members :NoStudent ID Name Mobile No.1 2019415918 Ratna Rabiah bt Gamel Abdul Nasseer 013-598 81422 2019475486 Noraisyah bt Zulkifli 017-3 2019685514 Mahani bt Othman 019-4 2019685484 Nur Ezzaty bt Mohd Badrul Isham 012-Practical 1 : Identification of Biosafety HazardsBMSBiorisk ...

Description

BMS533 Biorisk Management

GROUP :

AS201

TEAM :

Members :

N o 1

Student ID

Name

Mobile No.

2019415918

Ratna Rabiah bt Gamel Abdul Nasseer

013-5988142

2

2019475486

Noraisyah bt Zulkifli

017-4330274

3

2019685514

Mahani bt Othman

019-3936142

4

2019685484

Nur Ezzaty bt Mohd Badrul Isham

012-3144256

Practical 1 : Identification of Biosafety Hazards

MIC674 Biosafety and Biosecurity

GROUP : AS201

TEAM :

Practical 1

Submit one report per group

LEADER Members

: Mahani bt Othman : Ratna Rabiah bt Gamel Abdul Nasseer : Noraisyah bt Zulkifli : Nur Ezzaty bt Mohd Badrul Isham

Date

:

Report writing : This section

was written by

Introduction

:

Ratna Rabiah bt Gamel Abdul Nasseer

Methods

:

Nur Ezzaty bt Mohd Badrul Isham

Results

:

Mahani bt Othman

Discussion

:

Noraisyah bt Zulkifli

and reviewed by

Title : Identification Of Biosafety Hazards

Introduction Laboratory hazard is defined as anything that has a potential to cause damage. People working in the laboratory are exposed to potential hazards. Risks can be minimized by eliminating dangerous situations where possible, establishing clean and safe work habit, taking proper precautions at all times and maintaining awareness of good safety practices. Laboratory hazards should be taken seriously as you don’t want to mess with the lives of people and to avoid any dangerous situation in the lab. Most hazards encountered fall into three main categories: chemical, biological or physical.

Objective To identify possible biosafety hazards in a laboratory.

Materials and methods A layout map of the lab was drawn on a A4 paper. The layout must fit the paper to have a clear image. 5 hazards are identified in the lab. The harm that they would cause are stated in the result.

Results Figure 1.1 Layout of Lab

Figure 1 shows the layout of the lab 602. There are two doors to this lab. This lab consist of 1 lecturer’s bench and 4 working benches with one sink each. On the right side of the lab, there is a laminar flow and a chemicals rack. Meanwhiile, the left side of the lab is equipped with an incubator and a shaker. Near the lecturer’s bench, there is an autoclave on its left and a chemical bin on its right side. At the back of the lab, there is a microscopes’ rack and a shower. The safety features in this lab are the sinks, the shower and also the chemicals bin.

Table 1.1 List of hazards which are throw chemical waste in the sink, do not wear glove when handling chemicals, UV ray of laminar flow, Escherichia coli, Streptococcus aureus.

Hazard

Reason that it is a hazard

Harm that it can cause

1.

Throw the chemical waste in the sink

It could causes contamination to the water

Could lead to many diseases such as diarrhoea, cholera and typhoid fever

2.

Do not wear gloves when handling chemicals

It can cause infections and contamination and some chemicals are carcinogenic.

It can cause burn, rashes, allergies, and cancers.

3.

UV ray of laminar flow

Long exposure to UV rays

It could damage the eye and causes blindness.

4.

Escherichia coli

E.coli produces a powerful toxin that damages the lining of the small intestine

It could lead to diarrhoea.

5.

Streptococcus aureus

It can produce toxins

It could cause diseases such as toxic shock syndrome, food poisoning, and abscesses

Discussion

Biosafety is defined as, “The discipline addressing the safe handling and containment of infectious microorganisms and hazardous biological materials”. The practice of safe handling of pathogenic micro-organisms and their toxins in the biological laboratory is accomplished through the application of containment principles and the risk assessment. Risk assessment is the first component of the AMP model for biorisk management and also an integral part of laboratory biosafety. There are many hazards that can be found in the laboratory and may harm the users if not handling it with care. The most common mistake that students always do after the experiments is throwing the chemical waste directly into the sink, as it can caused contamination to the water and lead to many disease such as diarrhoea, cholera and typhoid fever. Next, the hazard could happen when handling the chemicals without wearing the gloves as it can cause infections, contamination and some chemicals are carcinogenic. The exposure of the strong chemical to the skin can cause burn, rashes, allergies and cancer. Direct contact with the UV rays of the laminar flow for a long time without using the google could damage the eye and may cause blindness. The biological agents that can cause hazard such as E. coli can cause diarrhoea can be transmitted through contaminated water or food, or through contact with animals or persons. E. coli produces a powerful toxin that damages the lining of the small intestine. Another biological agent that can be harmful is Streptococcus aureus, it can produce toxin and lead to many diseases such as toxic shock syndrome, food poisoning, and abscesses. Handling or using the autoclave are potentially highly hazardous and require special attention because it use high pressure and high temperature steam to kill microorganisms and render biohazardous material inactive. Potential risks of using an autoclave are heat and steam burns, hot fluid scalds, injuries to hands and arms from the door, and bodily injury in the event of an explosion. To prevent the injuries, wear appropriate Personal Protective Equipment (PPE) including a lab coat, heat resistant gloves, and eye protection, especially when unloading the autoclave.

Conclusion The objective is achieved as there are several biosafety hazard in the laboratory that have been identified which are throw chemical waste in the sink, do not wear glove when handling chemicals, UV ray of laminar flow, Escherichia coli, Streptococcus aureus.

References 1. E. coli. ( 2020, July 19). Retrieved from https://www.mayoclinic.org/diseases-conditions/e -coli/symptoms-causes/.syc-20372058 2. Lab Manager. 2020. Biosafety Levels 1, 2, 3 & 4. [online] Available at:

[Accessed 16 March 2020].

3. Drs.illinois.edu. 2020. - Division Of Research Safety | Illinois. [online] Available at: [Accessed 16 March 2020].

Lab Report Rubric PRAC # 1

(include this with every report)

Exceeds Expectations

Attribute Marks

5

Meet Expectations 3 --> 4 Submitted on time

Below Expectations

Mark s

Tot al

0 --> 2

1. Punctuality Was the report submitted on time?

Submitted early

Submitted late

2. Organization of report Is the prescribed format used?

Good, clear format

4. Introduction Did the students provide background materials?

Good, concise summary of background

OK. Materials included but not summarised or too long.

Inadequate summary /duplicated from sources.

5. Outcomes Hazard 1 Are both the hazard and possible risk

Well defined.

Acceptable, student is a bit unsure

Not well define. Student is unsure.

5. Outcomes Hazard 2 Are both the hazard and possible risk

Well defined.

5. Outcomes Hazard 3 Are both the hazard and possible risk

Well defined.

5. Outcomes Hazard 4 Are both the hazard and possible risk

Well defined.

5

Loose format

Not organized 5

5

5

Acceptable, student is a bit unsure

Not well define. Student is unsure. 5

Acceptable, student is a bit unsure

Not well define. Student is unsure. 5

Acceptable, student is a bit unsure

Not well define. Student is unsure. 5

6. Results Figure a. Is Figure in proper format?

Well formatted, clear

Proper format

Not formatted

7. Discussion Is a discussion inlcuded?

Results were well discussed.

Some attempts. mainly descriptive.

No attempt to discuss.

8. Conclusion Was a conclusion provided?

Correct conclusion, related to the objective

Conclusion is provided, but not directly related to the objective

No conclusion/ conclusion not related to objective(s)

5

SUM

50

Evaluated by

5

5

:_________________________________________ Date : ________________________...