Prelab Exercises for Gas Chromatography PDF

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Prelab exercise...

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Prelab Exercises for Gas Chromatography Name_ _____________________

Section____ON3_______________________

1. What is the purpose of this experiment? In this experiment, in order to determine the purity and identity of methyl and isobutyl alcohol Gas chromatography will be ensued.

2. In all types of chromatography, there are two types of phases involved in the technique. What are these two kinds of phases called? Mobile and stationary

Identify the two phases used in gas chromatography. -the stationary phase is a thin layer of solid material - the mobile phase is a liquid in which the mixture of interest is dissolved.

3. How do compounds become separated from each other as they pass through the GC? The different compounds in the sample move through the adsorbent at different rates due to their different physical properties and become separated.

4. What is the retention time? time taken for a solute to pass through a chromatography column

How can the retention time help you in determining which peak will correspond to methanol and which to isobutyl alcohol? A known compound that is believed to be one of the components of the original sample mixture may be injected into the GC and its retention time measured. This can serve as a reference and may provide evidence for the identification of the compounds in the sample.

5. What does the area of the peak tell you? amount of the compound that is present 6. What is the operating temperature range for the mini GC column?

30-160 degrees Celsius...