Report sheet CSrye PDF

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CSRye: Mascot Mystery REPORT SHEET

Tube #

Phosphate content (mg/10mL)

Phosphate concentration

Absorbance reading (%)

Absorbance Reading

1

0

0.00

100

2

0.01

1.00

80.2

3

0.02

2.00

62.5

1 log= =0.00 1 1 log= =0.0950 0.1802 1 =0.204 log= 0.625

4

0.03

3.00

44.1

log=

1 =0.355 0.441

5

0.04

4.00

39.2

log=

1 =0.407 0.392

6

0.05

5.00

29.9

log=

1 =0.524 0.299

7

Eggy’s lungs

1.38

71.4

log=

1 =0.146 0.714

8

U of T Pool

2.24

57.9

log=

1 =0.237 0.579

9

York Pool

2.83

50.2

log=

1 =0.299 0.502

10

Lake Scugog

1.47

70.0

log=

1 =0.155 0.700

To Calculate the phosphate calculation in test tube #3

equation of the graph= y=0.1059 x−0.0006 R2=0.9905 Substitute y in the equation of the graph with absorbance calculated above in the 5th column will result in the phosphate concentration in ppm y =0.1059 x −0.0006 0.204=0.1059 x−0.0006

0.204 + 0.0006 =x 0.1059 x=1.9 ≈ 2

Based on the above data, which suspect was the closest to the crime scene Lake Scugog.

The absorbance of phosphate vs concentration of phosphate in ppm measured using a spectomenter at 675 nm Absorbance U of T Pool

Calibration Line York Pool

Eggy's lungs Lake Scugog

0.6

f(x) = 0.11 x − 0 0.5 R² = 0.99

Absorbance

0.4

0.3

0.2

0.1

0 Concentration of phosphate (ppm)...