The Fastest Enzyme - Introduction PDF

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early draft on the experiment...

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Introduction Enzymes are protein that acts as biological catalysts. They will help to speed up the biochemical reactions in the cell but remain unchanged at the end of the reactions. Enzymes degrade molecules known as substrates. Each enzyme can only break down one type of substrate because each enzyme has a specific active site to bind to a particular substrate. Catalase is an enzyme that catalysts the decomposition of hydrogen peroxide into water and oxygen. Catalase, which is abundant in organisms that live in the presence of oxygen, prevents the accumulation of peroxide, which is continuously created by numerous metabolic reactions and protects cellular organelles and tissues from damage.

2𝐻2 𝑂2 ↔ (𝑐𝑎𝑡𝑎𝑙𝑎𝑠𝑒) ↔ 2𝐻2 𝑂 + 𝑂2 The activity of an enzyme is influenced by environmental factors such as temperature and pH, as well as chemicals that are unique to that enzyme. Each enzyme has a reaction rate that is best at a specific temperature. Enzymes work best at temperatures between 35 and 40 degrees Celsius. Each enzyme has a reaction rate that is best at a specific temperature which is known as optimum temperature. Enzymes work best at temperatures between 35 and 40 degrees Celsius (Campbell, et al., 2021) . Excessive heat can cause the enzyme to denature and lose its catalytic properties. Furthermore, cells often increase the rate of reaction by producing more enzyme molecules. It is because the reaction rate between an enzyme and substrate can be increased with constant temperature, constant pH, and sufficient substrate concentration. The objective of this experiment is to study the effects of high temperature on the catalase enzyme. The other purpose of this experiment is to study the effect of enzyme concentration on the rate of reaction. The questions are what are the effects of high temperature on the catalase enzyme and how enzyme concentration affect the rate of reaction. The hypothesis for the first objective is the high temperature will denature the enzyme. In which, the reaction will not occur. Next, the hypothesis for the second objective the higher the concentration of enzyme, the higher the reaction rate....