UNIT TASK #14 Preparation of Adhesives PDF

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UNIT TASK #14: Preparation of Adhesives, Fishing-Out of Paraffin Sections, and Drying of slidesAnswer the following.1. What is the use of thymol in the preparation of Mayer’s Egg Albumin? (2 pts)Thymol used to prevent the growth of molds in the preparation of Mayer’s Egg Albumin2. What will happen i...

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UNIT TASK #14: Preparation of Adhesives, Fishing-Out of Paraffin Sections, and Drying of slides Answer the following. 1. What is the use of thymol in the preparation of Mayer’s Egg Albumin? (2 pts) Thymol used to prevent the growth of molds in the preparation of Mayer’s Egg Albumin 2. What will happen if the temperature of the floatation water bath is lesser than its prescribed temperature? (3 pts) If it is too hot, desiccated-looking sections will result, while cool water baths will produce excessive wrinkling of the tissue. 3. What will happen if the section stays long in the water bath (2 pts) The section must not be left in the bath for more than 1 or 2 minutes, or over-hydration of the tissue will be produced, simulating the appearance of edema fluid when examined microscopically. 4.

Enumerate and describe the commonly used adhesives, and give their composition (8 pts)

Mayer's Egg Albumin- Mayer's egg albumin is the most commonly used because it is very easy to make, is convenient, and is relatively inexpensive. Dried Albumin - dried, and stored in 70% alcohol until it is ready for staining

COMPOSITION: Egg White 50 cc. Glycerin 50 cc.

COMPOSITION: Dried Albumin - dried, and stored in 70% alcohol until it is ready for staining Gelatin (1%) COMPOSITION: Gelatin 1 gm Distilled water 100 ml Glycerol 15 ml Phenol Crystal 2 gm Gelatin-formaldehyde mixture COMPOSITION: 1% gelatin 5 ml 2% formaldehyde 5 ml Poly-L-Lysin e- This is widely used as a section This aqueous detergent can be purchased as a adhesive in immunohistochemistry. 0.1% solution which is further diluted 1:10 with distilled water (final dilution to 0.01%) prior touse. APES (3-aminopropylthriethoxysilane)- very The slides are dipped in 2% APES in acetone, useful in cytology, particularly for cytospin drained, dipped in acetone, drained again, and preparations of bloody material finally dipped in distilled water. References: HISTOPATHOLOGIC TECHNIQUES JOCELYN H. BRUCE-GREGORIOS...