Activity 1.1.5 - Part I Student Response Sheet PDF

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Just being nice...

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Created by Crystal Ponticello, Ph.D. Adapted from © 2013 Project Lead the Way, Inc.

Activity 1.1.5: ELISA Part I: Principles of the ELISA Test A) Read through the introduction and procedure for Activity 1.1.5, then complete steps 1-2 independently. Write a paragraph that explains how antibodies work to keep us well in the space provided below. The following terms must be used and underlined in the paragraph: antigen, antibody, immune system, B lymphocytes (please use a different color font).

B) Watch the ELISA animation in step 3. Independently, use what you learned in the video to draw a labeled and colored sequential diagram or to construct a small interactive model sketch that shows how the ELISA test can be used to identify patients who are infected with bacterial meningitis. Your drawing or model should include the following labeled components: antigen, primary antibody, secondary antibody, enzyme, substrate, Neisseria meningitidis bacteria. Insert a picture of your diagram or model sketch here:

C) Complete the conclusion questions (please use a different color font): 

Explain why antibodies allow scientists to target and identify specific disease agents.

Medical Interventions Activity 1.1.5 ELISA – Page 1

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Why is the secondary antibody used in an ELISA test conjugated with an enzyme? What happens when this enzyme meets up with its substrate?

Medical Interventions Activity 1.1.5 ELISA – Page 2...