Animal and plant cell lab PDF

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comparison between animal cells and plant cells under different conditions ...

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COMPARISON OF ANIMAL AND PLANT CELLS

Comparison of animal and plants cells Yajaira Orellana September 24, 2020 Montclair State University

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COMPARISON OF ANIMAL AND PLANT CELLS

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Objective The objective of this laboratory was to observe and analyze how plant and animal cells reacted under different ionic conditions such as hypotonic, hypertonic and isotonic. At the same time, I looked at the similarities and differences among both types of cells.

Protocol Materials used to view plant cells ● Microscope slides ● Light microscope ● Coverslips ● Elodea leaves ● 5% NaCl ● Tap water ● Deionized water 1. For the plant cell observation, I used an Elodea sprig; in which I took 3 leaves to use it for the 3 different conditions I was testing 2. I started by putting the elodea leaf on the microscope slide, then adding 5%NaCl and covering the slide with a coverslip 3. Then I looked at the slide under the microscope under 3 different magnifications (4x, 10x and 40x) 4. After I took pictures for each magnification I made another slide but this time using Tap water for the environment

COMPARISON OF ANIMAL AND PLANT CELLS

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5. Then proceed to follow steps 3-4 6. Finally I made the last slide but using deionized water and looked at the slide under the microscope and took pictures to analyzed later on Materials used to view live animal cells ● Sterile swabs ● Microscope slides ● Coverslips ● Methylene blue ● Deionized water ● 0.9% NaCl ● 5%NaCl 1. Using a sterile Swab I scrubbed the inside of my cheek to get the epithelial cells of my mouth 2. Immediately I smear the swab on the microscope slide before it dried and added a drop of methylene blue dye because if not if would have been very difficult to look at the cells 3. Then I proceed to carefully cover it with a coverslip 4. I looked at the slide under the microscope in the 3 different magnifications, starting with the lowest one (4x) and going my way up to 40x 5. Next, using the swab I collected my cheek cells and smear the swab in a different microscope slide added the methylene blue but this time I added a drop of 5% NaCl 6. Then I followed the same procedure in step 3 and 4

COMPARISON OF ANIMAL AND PLANT CELLS

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7. I repeated the same procedure collecting my cheek cells and adding methylene blue but instead of adding 5%NaCl, I first added .09% NaCl and then deionized water. 8. Then I look at each slide under the microscope under the 3 different magnifications.

COMPARISON OF ANIMAL AND PLANT CELLS Results/Data Plant cell under 5% NaCl environment Plant cell under 4x magnification

Plant cell under 10x magnification

Plant cell under 40x magnification

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COMPARISON OF ANIMAL AND PLANT CELLS

Plant cells under Tap water environment Plant cell under 4x magnification

Plant cell under 10x magnification

Plant cell under 40x magnification

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COMPARISON OF ANIMAL AND PLANT CELLS Plant cells under deionized water environment Plant cells under 4x magnification

Plant cells under 10x magnification

Plant cells under 40x magnification

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COMPARISON OF ANIMAL AND PLANT CELLS Animal cell with methylene Animal cell under 4x magnification

Animal cell under 10x magnification

Animal cell under 40x magnification

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COMPARISON OF ANIMAL AND PLANT CELLS Animal cell with methylene under 5% NaCl environment Animal cell under 4x magnification

Animal cell under 10x magnification

Animal cell under 40x magnification

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COMPARISON OF ANIMAL AND PLANT CELLS Animal cell with methylene under .09%NaCl environment Animal cell under 4x magnification

Animal cell under 10x magnification

Animal cell under 40x magnification

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COMPARISON OF ANIMAL AND PLANT CELLS Animal cell with methylene under deionized water environment Animal cell under 4x magnification

Animal cell under 10x magnification

Animal cell under 40x magnification

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COMPARISON OF ANIMAL AND PLANT CELLS

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Discussion Osmosis is the movement of water from inside to outside the cell or vice versa, it happens when the solute cannot pass through a membrane but the solvent (water) can and it is caused to maintain the equilibrium of the cell. After I looked at my results, I did not really see a lot of difference in the way the cell looked overall, however the important concepts of the lab was to understand how animals and plant cells react differently to their environment depending on the surroundings. When I looked at the animal cells I could see how they reacted differently, when I added deionized water to the environment, the cell burst, this was caused by the hypotonic environment (water was moving inside the cell). At the at the same time, when I added 5% NaCl to the environment, the cell shrunk and spread out because the water diffused into the hypertonic solution (water was leaving the cell). Meanwhile when I added 0.09% NaCl the cell did not change at all, meaning the .09% NaCl solution did not affect the cell (the environment was isotonic). Similarly when I looked at the plant cells I also observed the different ways it reacted, when I added the 5% NaCl solution the plant cell become flaccid/shrunk the same way an the animal cell did, water was moving out the cell (water was diffusing into the hypertonic solution). Meanwhile when I added deionized water or tap water the cell become turgid caused by the hypertonic environment, (water was going inside the cell). I realized there are some similarities between both cells, they reacted similar to the 5%NaCl solution, both cells shrunk because their environment had a higher concentration of solute. On the other hand, they reacted differently to deionized water, the animal cell bursted, meanwhile the plant cell just became turgid. This is caused by the vacuole in plants, the elasticity of the vacuole helps the plant cells to get bigger, pushing the cell membranes against the cell wall, this created pressure that then prevents more water from coming into the cell. Animal and plant cells are similar yet very different at the same time, they have different organelles that help then go through the

COMPARISON OF ANIMAL AND PLANT CELLS different tasks and processes.

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