AP Bio Photosynthesis Lab PDF

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Photosynthesis Lab with data...

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Diana Bocina AP Biology Mr. Rath 12 November 2015 Photosynthesis- Floating Disc Lab Problem: How does CO2 affect the rate of photosynthesis in a spinach leaf? Hypothesis: If the spinach leaf is placed in the CO2 solution, the rate of photosynthesis will increase because photosynthesis depends on gas exchange, specifically CO2. Therefore, when the spinach leaf is placed in the CO2 solution, the rate of photosynthesis will increase due to gas diffusion. Materials: 20 spinach lead disks, 1 cup of CO2 solution, 1 cup of distilled water, 2 100mL syringes, 1 hole puncher, 1 stopwatch, 1 light source. Procedure: 1. Collect leaf disks by punching holes into spinach leaf, make 20 circles. 2. Make a solution of sodium bicarbonate by mixing 300Ml of water and a pinch of baking soda. 3. Place 10 leaf disks in the syringe and pull a small amount of CO2 solution. Replace the plunger and push out all the air. 4. Create a vacuum by putting your thumb and pinky around syringe and pull back on plunger. 5. Release vacuum to get disks to sink in the syringe solution. This may take a few times. 6. Once the disks have sank in the syringe, put the disks into the CO2 solution and start a timer with a light source. 7. Repeat set up from above but do not place baking soda in the beaker. This is the control 8. Both experimental and control group should run until all discs are floating to the top. Data Collection: Average Rate of Change for no CO2: (10-0)/ (15-0) = 0.67 disks/min Average Rate of Change for CO2: (10-0)/ (12-0) = 0.83 disks/min ET50 for no CO2: 9.5 minutes ET50 for CO2: 6 minutes...