Audar 2.3 LAB Report - laboratory experiments from labster in chemistry laboratory for engineering PDF

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1 st Quarter S. 2020-Experiment 2 Lab ReportEutrophicationSubmitted by:Audar, Ira Mikhail S.Submitted to:Ms. Marilen M. MartinSubject and SectionCM011L - FOPI1 st Quarter S. 2020-1. INTRODUCTIONWhenever the environment is becoming nutrient-rich, this is referred to as eutrophication. That could be a...

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School of Chemical, Biological and Materials Engineering and Sciences 1st Quarter S.Y. 2020-2021

Experiment 2.3 Lab Report

Eutrophication Submitted by: Audar, Ira Mikhail S.

Submitted to: Ms. Marilen M. Martin

Subject and Section CM011L - FOPI04

School of Chemical, Biological and Materials Engineering and Sciences 1st Quarter S.Y. 2020-2021

1. INTRODUCTION

Whenever the environment is becoming nutrient-rich, this is referred to as eutrophication. That could be a concern in aquatic ecosystems like lakes, where it can create algal blooms. Fertilizers are frequently employed in farming. Nevertheless, chemical fertilizers can occasionally run off towards surrounding water, producing an increase in nutrient concentration. This accelerates the growth and reproduction of microalgae, likely to result in algal blooms. This algal bloom disturbs the regular functioning of ecosystems and produces dozens of new issues. Microalgae might indeed consume all of the oxygen throughout the waters, leaving insufficient oxygen for several other aquatic life. Several marine species, including fish, die from complications because they rely on the oxygen in the water to survive. Algae blooms may also prevent sunlight from reaching maritime photosynthesis vegetation beneath the water's surface. Toxins produced by certain algae are hazardous to higher species of life. This could also disrupt the local ecosystem and harm any animal that consumes them. A. Brief Background ( 1 Paragraph or 5 sentences)

The simulation introduces on a fish farm, in which there was an unexplained massive cause of the death of the farm's fishes. The owner of the fish farm needs to investigate what was causing the deaths of several fishes. And we were tasked with investigating the big fish death scenario by examining the water sample from the fish farm as well as a sample of a dead fish. Overall following report would be on the eutrophication method.

Objectives: The objective of the simulation is to find the unexplained massive cause of the death of the farm's fishes.

School of Chemical, Biological and Materials Engineering and Sciences 1st Quarter S.Y. 2020-2021

2. ANALYSIS A mass killing of fish suddenly occured in Trisha's farm. We were asked for helped to investigate and experiment the cause why the fished died. At first we collected a sample of the surface of the water and a dead fish and put it inside a cooler to be brought to the laboratory to examine.

In the desk we have the water sample and the dead fish sample. We will examine it using a microscope and identify the organism that could be the cause. Process in preparing the water sample to be examined in the microscope.  First put a microscope slide on the bench  Second put a sample of the water from the farm using a pipette.  Third put a cover glass on the specimen.  Lastly put it in the microscope to examine it.

School of Chemical, Biological and Materials Engineering and Sciences 1st Quarter S.Y. 2020-2021

Repeat the process in preparing the sample from the dead fish to be examined in the microscope.

 First put a microscope slide on the bench  Second grab the scrapel and take a scale sample from the dead fish.  Third put a sample of the water from the farm using a pipette.  Fourth put a cover glass on the specimen.  Lastly put it in the microscope to examine it. After examining it the type of organism found on the water and the scale of the dead fish what is found is a Toxic Microalgae. Microalgae are minute algae that are undetectable to the naked human eye. They are phytoplankton that lives between the aquatic environment and the substrate of marine and terrestrial environments. They are single-celled organisms that can live alone. Its diameters can vary from several micrometers to about 100 micrometers depending on the variety.

The process in growing an algae in large amount is through nutrients. We now have a lead in finding the reason behind Trisha's farm crisis. Now we will analyze and examine the water samples where the nutrients surplus originates. We took six samples from different location of the surroundings of the farm. The Map where we took some water samples.

School of Chemical, Biological and Materials Engineering and Sciences 1st Quarter S.Y. 2020-2021

Now we will proceed in using the spectrophotometer.

School of Chemical, Biological and Materials Engineering and Sciences 1st Quarter S.Y. 2020-2021

In the desk we have a cuvette that has a blank solution in it. Before testing some samples we will first measure the blank cuvette.

 First we will pick up the blank cuvette  Second we will put it inside the spectrophotometer and press blank. Now after we have done measuring the blank cuvette we will now measure the water samples we collected.

 First it to pick up three cuvette  Second we will use the pipette to transfer the sample water to the cuvette.  Third we will take sample 2, 4, and 5 and transfer it to the cuvette.  Lastly is put each cuvette in the spectrophotometer to know the amounts of each sample.

The results is:  Sample 2 - Nitrogen concentration = 0.5 mg/l  Sample 4 - Nitrogen concentration = 0.7 mg/l  Sample 5 - Nitrogen concentration = 1.8 mg/l So we concluded that the culprit of the cause is Alex's Cotton Field. 3. CONCLUSION

School of Chemical, Biological and Materials Engineering and Sciences 1st Quarter S.Y. 2020-2021

The conclusion of the simulation investigation through the experiment is that the cause behind the deaths of many fishes from Trisha’s farm is the toxic algae in her farm. The cause of the toxic microalgae spreading is the nutrients spreading in the water surrounding. The culprit we concluded is Alex’s Cotton Field. Alex uses nutrients or fertilizers to grow his cotton field. The causes the spread in trisha’s farm. The objective is met in the experiment we were able to find the culprit and the cause behind the crisis. REFERENCES: https://www.water-pollution.org.uk/eutrophication-and-water-pollution/ https://www.sciencedirect.com/topics/earth-and-planetarysciences/eutrophication...