Basic Microscope - o Total magnification of specimen = objective lens power x eyepiece (always PDF

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o Total magnification of specimen = objective lens power x eyepiece (always 10x)...

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DISSCUSION Parts and Functions  Eyepiece = allows you to view the specimen on the stage o Ocular lens = magnification of 10x  Body tube = maintains distance between the eyepiece and the objective lens o Allows light to pass up to the eyepiece to view the specimen  Arm = allows you to carry the microscope o Supports the upper portion of the microscope  Revolving nosepiece = allows you to turn and change objective lenses  Objective lens = magnifies the specimen o Low power = 4x (see more of the specimen) o Middle power = 10x o High power = 40x (allows to see more detail) o Total magnification of specimen = objective lens power x eyepiece (always 10x)  Stage = holds the slide to view the specimen o Opening allows for light to pass through  Stage clips = holds the slide in place o Do not bring in contact with the opening of the stage  Diaphragm = adjusts the amount of light coming through the stage  Coarse adjustment knob = large knob on the side of the arm that physically moves the stage to focus on a specimen o Always use for low and middle power objective lenses  Fine adjustment knob = small knob below the coarse adjustment knob that slightly sharpens the image of your specimen o Always use for only high power objective lens  Light source = source of light to view your specimen  Base = allows you to carry the microscope o Supports the microscope

Basic Microscope Technique Rules to Follow • 1. If you must carry a microscope, always hold it with one hand on the arm and the other under the base. • 2. Always lower the stage or raise the objectives all the way before placing a slide under the objectives. • 3. Always begin working with the LOW POWER (shortest) objective first. • 4. Observe the slide from the side, not looking through the eye piece, when using the coarse focus to avoid running the objective lens into the slide. • 5. Never use the coarse focus adjustment when on the medium or high power objectives. Focus on low power first and then rotate the higher power objective into place. Make final focus adjustments with the fine focus adjustment.

To calculate the power of magnification, multiplythe powerof the ocular lensby the powerof the objective.

o Total magnification of specimen = objective lens power x eyepiece (always 10x)

Example : How to make wet-mount slide… 1- Get a clean slide and coverslip from lab technician 2- Place ONE drop of water or immersion oil in the middle of the slide . Don’t use too much or the water / immersion oil will run off the edgeand make a mess! 3- Place the edge of the cover slip on one side of the water drop / immersion oil 4- Slowly lower the cover slip on top of the drop

5- Place the slide on the stage and view it first with the red-banded objective. Once you see the image, you can rotate the nosepiece to view the slide with the different objectives.

Conclusion The microscope compound , often referred to as the light microscope, is a type of microscope that commonly uses visible light and a system of lenses to magnify images of small objects. Microscopes compound are the oldest design of microscope and were possibly invented in their present compound form in the 17th century. Basic optical microscopes can be very simple, although many complex designs aim to improve resolution and sample contrast. Often used in the classroom and at home unlike the electron microscope which is used for closer viewing.

Reference https://www.microscopemaster.com/...