Bio lab 1 - Stomata lab PDF

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Stomata lab...

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Bi o4 St oma t al a b 1/ 30/ 18

H0:nul lh y po t h e s i s : Th e r ewi l lbenos i g ni fic a ntdi ffe r e n c ei ns t oma t ad e n s i t ybe t we e nho l l yl e a v e sgr o wi n gon at r e ei nt hes ha dea n dh ol l yl e a v e sgr o wi n gonat r e ei nt h es u n.

Stomatal Density (#/mm2)

Ha:a l t e r na t eh yp ot he s i s Th e r ewi l lbeas i g ni fic a ntdi ffe r e n c ei ns t oma t ade n s i t yb e t we e nho l l yl e a v e sgr o wi n gon at r e ei nt hes ha dea n dh ol l yl e a v e sgr o wi n gonat r e ei nt h es u n.

200 180 160 140 120 100 80 60 40 20 0

SHADE _x000d_(n=258) Light Intensity Fi g ur e1 . St oma t a ld e n s i t i e s( s t o ma t a / mm2)o fAme r i c a nHo l l y( I l e xo pac a)l e a v e sob t a i ne di n l a t es u mme ra nde a r l ywi n t e rf r o m2 01 3t hr ou g h2 018, f r o m ma t u r e , s u ne xpo s e d( n =86l e a v e s , 3r e pl i c a t evi e ws )a n ds ha de e x pos e d( n=8 6l e a v e s ,3r e p l i c a t evi e ws )t r e e sont hec a mp uso f Ro wa nUn i v e r s i t y , NJ .

St ude ntt t e s tr e s ul t s :

p value

p value

0.423

Two Tailed

Co nc l us i on Th et wot a i l e dt t e s tr e s u l t e di nt h epv a l u ebe i n gc a l c u l a t e dt obe0. 423 . Thi sc a l c u l a t e dp v a l u ei sgr e a t e rt ha nt hea l ph apv a l ueof0. 0 5. Fr o mt hi s ,wef a i l e dt or e j e c tt hen ul lh ypo t he s i s a ndc a nn ota c c e p tt hea l t e r na t eh ypo t he s i s . Th en umb e ro fs t o ma t ap e rm2h a sn os i gni fic a nt d i ffe r e nc eo nt r e e sgr o wi n gi nt hes una n dt r e e sgr o wi n gi nt hes h a d e . 1. On ee xpe r i me n t a ld e s i g nfla wi sp s e udo r e pl i c a t i o nbe c a u s ema n yl e a fs a mpl e swe r e t a k e n, b utl e a v e swe r eonl yt a k e nf r omt wot r e e sonc a mpus . Ab e t t e rde s i gnwoul db et o t a k el e a fs a mp l e sf r omma n yt r e e si nt hea r e a . Con f ou ndi n gv a r i a bl e si sa n ot he rde s i gn fla w. St oma t ad e n s i t ywa son l ys t udi e di nr e l a t i o nt os un l i ghtb utde n s i t yma ya l s obe i n flue nc e db ys oi lwa t e rc o nt e nt .Tofixt hi s , s a mpl e so fs oi lc oul dbet a k e na n dt hewa t e r c on t e n twoul db eme a s ur e d . Thel a s td e s i g nfla wi sbi a sbe c a us ed a t awa shandpicked. The leaves were hand picked off of the trees and stomata were counted by eye. 2. St oma t aa r egua r dc e l l st ha ts we l la nds hr i nki no r de rt oope na ndc l os et hepor e so na p l a n t sl e a f . Ra t he rt h a nj us tgr o wi n gbi g g e r ,t h e s egua r dc e l l smul t i p l y . Thi sr e s ul t si na v a r yi n gs t oma t ad e n s i t ya mo n g s tp l a n t sofd i ffe r e nte n v i r on me nt a lc on di t i o ns .

3 .St oma t ao c c urmo s t l yo nt heund e r s i de sofl e a v e st obes hi e l d e df r o mt h es una n dhe a ti t g i v e so fft oa v oi dgr e a ta mou nt soft r a ns pi r a t i ont ooc c ur . Pl a n t st ha ts h o wt h er e v e r s e p a t t e r na r eu s u a l l ypl a nt sf r o ma q ua t i ce n vi r o nme nt s( La wr e nc e ) . 4 .The r ea r egr e a t e ro z onec onc e n t r a t i o nsi nr u r a la r e a st h a nu r ba na r e a s( Al l e n ) . Pl a n t si n r u r a la r e a swo ul dha v eas ma l l e rs t oma t ade ns i t yt ha npl a nt si nur b a na r e a s . Asa nur b a n f a r me r , mypl a nt swo ul dr e q ui r egr e a t e ra mou nt so fwa t e rb e c a u s eo ft hegr e a t e ra mo unt o fs t o ma t ar e s u l t i n gi nagr e a t e ra moun toft r a ns pi r a t i on. 5 .St oma t ad e ns i t yma ys e r v ea sabi o i ndi c a t o rf ore s t i ma t i n gCO2c on c e nt r a t i onsi nt hep a s t b e c a us et h es t oma t ade n s i t yd e p e n dsont hea mou ntofCO2c on c e nt r a t i onsi nt he e n vi r o nme nta tt het i meoft hepl a n t sl i f e .Thegr e a t e ra mo un to fCO2a v a i l a bl e ,t h el o we r t hes t o ma t ad e n s i t ybe c a u s ep l a n t sc a nob t a i nal a r g ea mo un to fCO2wi t hl e s ss t oma t a . Whe r e a s , i ft h e r ei sal o wa mo un to fCO2a v a i l a bl e , pl a nt swi l lh a v eagr e a ts t oma t a d e n s i t yi no r de rt oo bt a i na ne ffic i e n ta moun to fn C e O e de df orc e l l ul a rpr o c e s s e s . 2

Wor k sCi t e d

1 .Al l e n, J e a nni e .“ TheOz oneWeBr e a t he:Fe a t ur eAr t i c l e s . ”NASA,NASA, Apr .200 2, e a r t hobs e r v a t or y . na s a . g o v/ Fe a t ur e s / Oz one We Br e a t he / .

2. La wr e nc e ,Loi s .“ Wh yDoWa t e rPl a nt sHa v eSt oma t aonUppe rPa r to fThe i rLe a v e s ?| Hu nke r . ”Hunk e r . c om,Hu nke r ,17J ul y20 17,www. hu nk e r . c om/ 134285 58/ wh y dowa t e r pl a nt s ha v e s t o ma t a onupp e r pa r t of t he i r l e a v e s ....