Biochemical Test PDF

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Summary

Lab Sheet Biochemical Test...

Description

MICROBIOLOGY LABORATORY Names of members ____________________ / ____________________ (Surnames only) ____________________ / ____________________

Date __________ Section _________

Group Number ____

Gram stain reaction

n/a

Cell shape Colony color on NA

n/a n/a

BIOCHEMICAL TESTS(Results should be entered as + / - only) Lactose fermentation MacConkey Agar Lactose fermentation with green Eosin Methylene Blue Agar metallic sheen Mannitol fermentation Mannitol Salt Agar Starch hydrolysis Starch Agar Gelatin hydrolysis Nutrient Gelatin Indole production Tryptone Broth Methyl Red test MRVP Broth Voges-Proskauer test MRVP Broth Citrate utilization Simmon’s Citrate Agar Glucose fermentation only (K/A) Triple Sugar Iron Agar Glucose and lactose/sucrose (A/A) Triple Sugar Iron Agar Non-fermenter (K/NC) Triple Sugar Iron Agar Gas production (with gas pockets) Triple Sugar Iron Agar H2S production (with blackening) Triple Sugar Iron Agar Motility MIO medium Indole production MIO medium Ornithine decarboxylase MIO medium Nitrate reduction Nitrate Broth Catalase test (culture from NA or SA) Oxidase test (culture from NA or SA)

Positv e Rod Slightly Yellow + + + + + + + + + + + + -

knownAssigned

ytoca Klebsiella

oniae Klebsiella

escens Serrata

aPseudomonas

isEnterococcus

Bacillus subtlis

usMicrococcus

MORPHOLOGY

Staphylococcus

Medium used

es Enterobacter

Morphological characters or name of biochemical test

Escherichia coli

Morphological and Biochemical Profiles of Selected Reference Organisms and Assigned Unknown...