Extra Biochemical Calculation Questions PDF

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Extra Biochemical Calculation Questions...

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1. What amount or quantity does 50µl of the following solutions contain? a. b. c. d. e.

10mM 5M 7mg/mL 8nM 11µM

2. Express the following concentrations with ‘ml’ as the volume term a. b. c. d. e.

4µg/µl 5mg/L 35ng/µl 28mg/µl 2kg/µl

3. A protein solution of unknown concentration was assayed as follows: 250µl of the unknown was taken and diluted to a volume of 1ml. An assay was then performed on 500µl of the diluted sample, which was found to contain 50µg of protein. What was the original concentration (in mg/mL) of the unknown protein solution? 4. 200µl of albumin solution of unknown concentration was diluted to a volume of 2mL using an appropriate buffer. 100µl of this diluted solution was transferred to a micro titer plate and made up to a final volume of 300µl with aqueous ammonia. Using a standard curve the well was found to contain 200nmol of albumin. What was the concentration of the original undiluted albumin solution in ‘mM’ units? 5. Express the following in nmol/µl a. b. c. d. e.

5mM 7µmol/ml 17mmol/L 10µmol/µl 15mM...