Biology lab report - Cellular Membrane Effect of Physical Stress PDF

Preview

Summary

Cellular Membrane Effect of Physical Stress ...

Description

Cellular Membrane Effect of Physical Stress Sabrina Pietrovicci Professor Wambugu Exercise 10 November 10th 2017

Abstract: During this lab my lab group and I worked on the effects of physical and chemical membranes. During this procedure we will be able to relate membrane structure to its function. Along with predict the effect of common organic solvents and extreme temperatures on membrane integrity and than calculate our results using beets throughout the experiment to use its membranes to see their general structures and functional membranes. So by using cold and hot temperatures including 70,55,40,22,5,and -5 degree Celsius water we were able to we will be able to find the differences in each membrane of a beet to find our conclusion. We used the table shown below and wrote down our answers on our lab sheet that was provided for us. So, if the extreme temperature causes damage to the cellular membrane than the 70 degree Celsius tube and the -5 degree tube Will provide the most damage.

Objective: The objective of this lab is to observe the membrane affected by stress. We will be describing aspects of the membrane structure to figure out if it is vulnerable to physical and chemical stress.

Procedure: During this procedure we will be observing the effect of temperature stress on cellular membrane. In the beginning of our lab we started off with 10.1, which wanted us to acknowledge the treatments to see what causes the most and least damage and

complete a chart of our results. The first thing we did was cut out six strands of beet using cork borer and than measure each piece to be exactly 15 mm long. My group and I than placed all the beet pieces into a cylinder to wash them off for 2 minutes to get the betacyanin from the injured cells on the surface. We than slowly placed each piece of beet into six individual test tubes and labeled them 1-6. 

Cold treatmentTo find out if the beet is good in cold temperature we placed tube 5 in the

refrigerator, which was approximately 5 degrees Celsius and placed tube 6 in the freezer at -5 degrees Celsius. We left both those tubes in there for 30 minutes, after 30 minutes we removed both tubes out of the cold and added 10ml of water into each tube and let it soak for 20 minutes. 

Hot TreatmentFor this part of the lab we took test tube 1 and placed hot water in the tube and let it stand in 70 degrees Celsius water for 1 minute. After 1 minute we placed 10.0 ml of distilled water into the test tube and let it sit. Than, we placed test tube 2 in 55 degree water for 1 minute and than let it sit in 10.0 ml of water after as well. For test tube 3 we did the same steps as test tubes 1 and 2 but placed it in 40-degree water. For test tube 4 we placed it in room temperature water for 1 minute than, added the distilled water to it as well. We than allowed test tubed 1-4 to soak in the distilled water for 20 minutes.

For all the test tubes, we than removed the beets from the tubes and measured the extent of the membrane injury following with the amount of betacyanin that is shown in the

water. We examined each test tube by examining the color of each between 0-10 which between colorless or darkest red. My group and I than wrote down our results on the graph provided for us during this lab. After looking at our results we placed each test tube into a spectrophotometer to read the absorbance of the solutions and wrote down our results for that as well. After getting all of our results we than created graphs to show the differences between each solution. Resuls Conclusion: To conclude our results, by looking at our calculation we came to an agreement that the freezing tube showed the most damage and the room temperature tube caused the least. By looking at the results from the spectrophotometer we saw that the hotter temperature caused more damage du to the leaking from the beet cells, but when measured in the machine the data was different and we were having multiple errors but were still able to find good results. Looking at my hypothesis, it was definitely not completely supported by the data obtained because it was predicted that the heat would do the most damage along with the freezing tube but it was mainly freezing that caused more.

References:...