Bisc 131 Enyzme Lab - Answers to Bisc 131 Enzyme Lab PDF

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Answers to Bisc 131 Enzyme Lab...

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Part 1: Pre-Lab Reading: Use your Pre-Lab reading materials to answer the following questions.

1. T/F: Chemical reactions in living things are sometimes destructive. (1) True

2. What is the purpose of a catalyst? (1) To speed up a chemical reaction

3. Why are catalysts important? (1) For cells to stay alive chemical reactions must occur. On their own, these reactions are slow, however with catalysts they are sped up and therefore the cell stays alive.

4. What is the name for biological catalysts made of protein? (1) Enzymes

5. What is the name for the starting materials of a chemical reaction? (1) Substrates

6. What are two ways that chemical reactions work on substrates? (2) The two ways that chemical reactions work on substrates include breaking them apart into multiple molecules or they will create a chemical bond between two substrate molecules.

7. At what point on the enzyme does a substrate bind? (1) The substrate binds to the active site of an enzyme

8. T/F: The active site of an enzyme is nonspecific to substrates. (1) False

9.

Why does the enzyme undergo a conformational change when binding the substrate? (1) Enzymes undergo a conformational change in order to make a more specific fit for the substrate for the chemical reaction to occur.

10. What is formed from the stress that an enzyme puts on a substrate? (1) The products of the chemical reaction are formed from the stress.

11. T/F: Enzymes can only bind substrates one time before the enzyme is no longer functional. (1) False

12. What is the substrate of the enzyme catalase? (1) Hydrogen Peroxide is the substrate of the enzyme catalase.

13. What are the products of the reaction of catalase with its substrate? (2) Water (H2O) and Oxygen gas (O2) are the products of the reaction. 14. Why is catalase important in the biological function of living things? (1) Certain catalysts such as Hydrogen Peroxide typically break down very slowly and when this happens it creates oxygen free radicals than can be harmful to cellular components. Catalysts are important because they speed up these potentially dangerous chemical reactions into products that are not dangerous.

Part 2: Answer the questions below using the information from this site (the site should automatically scroll down to Enzymes: Slide 4: Factors Affecting Enzyme Activity). http://www.bch.cuhk.edu.hk/vlab2/index.html#2/slide4

15. Name five things that influence reactions rates. (5) Temperature, pH, enzyme concentration, substrate concentration, and ionic strength are all factors that can influence reaction rates.

16. Click “Enzyme Concentration.” Increasing the enzyme concentration increases the ________. (1) a.

Amount of substrate

b.

Product concentration

c.

Both a & b

d. None of the above

17. Click the “...