[Bulletin of the Veterinary Institute in Pulawy] Determination of Zeranol and its Metabolites in Bovine Muscle Tissue with Gas Chromatography-Mass Spectrometry PDF

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Bull Vet Inst Pulawy 56, 335-342, 2012 DOI: 10.2478/v10213-012-0059-4 DETERMINATION OF ZERANOL AND ITS METABOLITES IN BOVINE MUSCLE TISSUE WITH GAS CHROMATOGRAPHY-MASS SPECTROMETRY

IWONA MATRASZEK-ŻUCHOWSKA, BARBARA WOŹNIAK, AND JAN ŻMUDZKI Department of Pharmacology and Toxicology, National Veterinary Research Institute, 24-100 Pulawy, Poland [email protected] Received: June 19, 2012

Accepted: September 7, 2012

Abstract This paper describes the quantitative method of determination of chosen substances from resorcylic acid lactones group: zeranol, taleranol, α-zearalenol, β-zearalenol, and zearalanone in bovine muscle tissue. The presented method is based on double diethyl ether liquid-liquid extraction (LLE), solid phase extraction (SPE) clean up, and gas chromatography mass spectrometry (GCMS) analysis. The residues were derivatised with a mixture of N-methyl-N-trimethylsilyltrifluoroacetamide, ammonium iodide, and DL-dithiothreitol (1,000:2:5, v/w/w). The GC-MS apparatus was operated in positive electron ionisation mode. The method was validated according to the European Union performance criteria pointed in Decision Commission 2002/657/EC. The average recoveries of all analytes at 1 µg kg-1 level were located between 83.7% and 94.5% values with the coefficients of variation values...