Cellular respiration project PDF

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Summary

a homework assignment based on our knowledge of cellular respiration...

Description

Cellular respiration

control (ml)

Pos. control (ml)

DNP (ml)

Cyanide (ml)

Arsenate (ml)

Glyphosate (ml)

1

0.5

5.5

2.2

2.7

1

4.4

2

0.5

5.1

2.1

3

1.2

5.8

3

0.5

4.1

1.8

2.8

1.1

4.4

Average

0.5

4.9

2.03

2.8

1.1

4.9

Trial

Neg.

1. Create an appropriately labeled bar graph for these results. Include positive and negative controls.

Neg. control

Pos. control

DNP

cyanide

arsenate

glyphosate

2. Answer the following questions about the cellular respiration experiments: a. Which of the above are inhibitors of cellular respiration? Use complete sentences and the data obtained from the experiment to support your answer. DNP, cyanide, and arsenate are inhibitors of cellular respiration because their values vary between the positive and the negative control with 2.03ml, 2.8ml and 1.1ml. b. Which of the above are not inhibitors of cellular respiration? Use complete sentences and the data obtained from the experiment to support your answer. Glyphosate has the same results of 4.9ml as the positive control, meaning it had no effect on cellular respiration. c. Why do you think the negative control still produced some carbon dioxide? When oxygen and yeast combine, aerobic respiration takes place and as a result, carbon dioxide is produced.

Fermentation

Trial

Neg. control

Pos.

DNP

Cyanide

Arsenate

Glyphosate

control

(ml)

(ml)

(ml)

(ml)

(ml)

(ml)

1

0.2

4.2

4.3

4.1

0.5

4.2

2

0.3

4.3

4.2

4.7

0.5

4.7

3

0.3

4.8

4.1

4.6

0.4

4.4

Average

0.3

4.4

4.2

4.5

0.5

4.4

1. Create an appropriately labeled bar graph for these results. Include positive and negative controls.

Neg. control

Pos. control

DNP

cyanide

arsenate

glyphosate

2. Answer the following questions about the cellular respiration experiments: a. Which of the above are inhibitors of fermentation? Use complete sentences and the data obtained from the experiment to support your answer. The inhibitor is arsenate, because its results were the closest to the negative control, 0.5ml to 0.3ml. b. Which of the above are not inhibitors of fermentation? Use complete sentences and the data obtained from the experiment to support your answer. Glyphosate has the same result as the positive control, 4.9ml. DNP and cyanide come very close to the positive control value, with values of 4.2ml and 4.5ml which means they are not inhibitors. Putting it all together 1. Were any of the above not inhibitors of both cellular respiration and fermentation? Glyphosate wasn’t an inhibitor to both cellular respiration and fermentation. 2. Were any of the above inhibitors of glycolysis only? Use your knowledge of cellular respiration and fermentation and the data obtained from the experiment to support your answer. No, none of them were inhibitors of glycolysis only. 3. What part(s) of cellular respiration do you think DNP and cyanide inhibit? Use your knowledge of cellular respiration and fermentation and the data obtained from the experiment to support your answer. DNP inhibits ATP from being produced. It uncouples the electron transport chain from ATP synthesis and because the chain can no longer form a proton gradient, and ATP synthase can’t make ATP.

Cyanide inhibits complex IV of the electron transfer chain in oxidative phosphorylation. Cyanide inhibits cytochrome c oxidase in the mitochondria, as a result, oxygen can’t be used and the electron transport chain stops working....