Digestion Lab - (Physio Lab) PDF

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Digestion Lab - Physio Lab...

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Physio Lab Esther Roldan Digestion Lab Exercise 1: 1. In which tube(s) did you noticed a color change? Explain your results/ Were these the results you expected to see? There was a color change in tubes 1 and 3. This is what my group mates and I had originally predicted considering the contents in each tube. When acidic, the color change is pink. Considering this, tube 1 and 3 turned pink because there was a presence of pancreatin which is responsible for emulsifying fats, which essentially leaves the solution acidic. However, tube 3 was slightly more pink than tube 1 since it contained bile salts which helps to break down the pancreatin even further.

2. Why would the color change to pink in tubes in which the reaction was occurring? A color change of pink indicates that the solution is acidic. This makes sense considering that the reaction that occurred in the tubes that turned pink contained pancreatin and were emulsifying fats.

Exercise 2: 3. Explain your observed results. Were they what you expected? Tube 1 contained starch, 2.5 mL of water and iodine while tube 2 contained starch, 2.5 mL of saliva and iodine. Based on these contents, tube 1 turned dark purple and tube 2 remained clear/cloudy. The results were as expected since in the presence of starch, iodine will turn purple. In tube 2, the saliva broke down the starch so the iodine had no starch to detect. However, in tube 1, there was left over starch in which the iodine could detect, therefore, turning purple.

Exercise 3: 4. Thoroughly explain how you were able to assign tube numbers to each list of contents. Test tube #4 matched with the contents of boiled egg white, 5 ml 0.1M HCl and 5 ml water considering that all the egg whites were still present in the tube. This was the only tube that did

not contain pepsin, therefore, there was nothing to breakdown the egg. Test tube #3 belongs to boiled egg white, 5 mL of 5% pepsin solution and 5 ml water. Considering that water was present in this test tube, the solution was neutral for the most part so there were pieces of the egg still present. As for test tube #1, it belonged to the tube containing boiled egg white, 5 ml of 5% pepsin and 5 mL 0.1M HCl. Considering that pepsin was present, it broke down the egg white, however, with HCl being present, the egg whites were further digested since HCl is acidic. Lastly, tube #2 matched with boiled egg white, 5 mL of 5% pepsin and 5 mL 0.1M NaOH as there were no egg pieces present which means the NaOH and pepsin had digested majority of the egg....