DNA Electrophoresis Results PDF

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DNA Lab Complete...

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DNA Electrophoresis See the lab manual, page 12-5 for the gel electrophoresis to answer questions 14. 1. Which suspect’s DNA fragments match the crime scene DNA fragments? S3 Use the DNA fragment bands (A, B, C, D, E) in lane S4 to answer questions #2-3 2a. Which band represents the longest DNA fragments? S1 2b. Which band represents the shortest DNA fragments? S1 3a. Which band represents the most abundant DNA fragments? S4 3b. Which band represents the least abundant DNA fragments? S2 4. What is the purpose of staining the agarose gels? To make it visible 5. Describe the role of PCR (Polymerase Chain Reaction) in DNA technology. Allows humans to make copies of existing DNA 6. Describe the role of restrictive endonucleases have in DNA technology. Cuts the DNA is half 7. Why do the DNA fragments move through the gel when an electric current is applied? Fragments are negatively charged attracting them to the postive side

Nucleic Acids 1a. What is the monomer of nucleic acids? Nucleotide 1b. What are the components of this monomer? pentose sugar, phosphate group, and nitrogenous base.

2. List three differences between RNA and DNA. DNA has thymine while RNA has uracil, dna is a double helix RNA translation DNA performes transition 3. What is the complementary base pair for this strand of DNA: ATCCTGAAG Tagga c tt C 4. If a DNA sample contains 14% cytosine, what are the percentages of each remaining nitrogen bases? 86%

DNA Extraction from Strawberries 1. Upload a photo of the DNA you extracted from the strawberries (included name in photo). 2. Why are strawberries a good choice to use for DNA extraction? The cell walls break down easily when mashed. Strawberries have 8 copies of each chromosome making them more plentyful....