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Enzymes Worksheet Group members: Santina, Lauren, Ekager & Alisha

Table 1 Enzyme concentration Time (seconds) A (low) B (medium) C (High)

Absorbance readings at each time interval (seconds) 0 0 0 0

20 0.221 0.336 0.610

40 0.406 0.631 1.083

60 0.566 0.854 1.444

80 0.676 1.053 1.722

100 0.802 1.223 1.927

120 0.916 1.411 -

Fig. 1 Graph of Absorbance Readings of the Enzyme Peroxidase Activity at Different Time Intervals (20 second intervals), at increasing enzyme concentrations of low, medium and high.

Fig .2 Graph of reaction rates from previous absorbance vs. time trials (see figure 1), at increasing concentration.

Table 2 Test tube # 1 2 3 4 5

Amount of substrate 0.5 1.0 2.0 4.0 8.0

Molecules of end product formed per minute (x 106) 72 145 300 350 350

Examine the trend in your data. Initially, as substrate concentration doubles, what happens to the reaction rate (Tubes 1-3)? As substrate concentration doubles the rate of reaction increases because the colour intensity starts to increase from tube 1 to 3 which indicates that there are more enzymes present and this increases the rate of reaction.

How would you explain the change that occurs at Tube3-4, and 4-5? The colour of the substrate intensifies because more substrate is being oxidized.

Considering these results, think back to exercise 1. If we had had you do a fourth set of tubes doubling again the concentration of enzyme, what do you predict might happen to the reaction rate? Do you think it would double again? Why/why not? As the concentration of enzymes doubles the reaction rate would double again because enzymes create chemical reactions that speed up the reaction rate. Therefore, increasing the amount of enzymes present, would increase the rate of reaction.

Table 3 Test tube # 1 2 3 4 5

pH 3 5 7 9 11

Molecules of end product formed per minute (x 106) 19 39 72 45 24

Based on the results above, what is the optimal pH for this enzymes function? The optimal pH for this enzymes function is 7 as it yielded the highest rate of reaction. How would you modify the above experiment to further narrow down the optimal pH? What range of pH would you test? I would test a smaller range of pH’s, such as a pH of 6-8 while increasing by 0.1 increments. This would allow me to identify which pH would be the exact perfect one for enzyme activity and narrow it down to one more exact then just 7....