GE Complementation test Q&A W2 PDF

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Summary

Genetics and Evolution worksheet with answers...

Description

Genetic Analysis: Complementation tests Antirrhinum majus is susceptible to infection by the Puccinia antirrhini fungus. You are interested in studying the infection process of this pathogen. From a mutagenesis screen, you identify individual plants that are resistant to infection. You identify ten mutants that cannot be infected by the fungus. In order to work out whether the mutations affect the same gene or different genes, you perform an F1 complementation test. To do this you cross the mutants with each other and test the progeny for resistance (R) or susceptibility (S) to the fungus. The results of this analysis are given in the table below. Lines

WT

1

2

3

4

5

6

7

8

9

10

WT

S

S

S

S

S

S

S

S

S

S

S

R

S

R

S

S

S

R

S

S

S

R

S

S

R

R

S

R

S

S

R

S

S

S

R

S

S

S

R

S

S

S

S

R

S

R

R

S

R

S

S

R

S

R

S

S

R

S

S

S

R

S

S

R

S

1 2 3 4 5 6 7 8 9 10

a) Why were all the mutant lines crossed to wild-type? To confirm that all the mutations were recessive b) How many complementation groups have been identified? four d) How many alleles have been identified in each complementation group? 1, 3, 7 (three), 2, 5, 6, 8 (four), 4, 9 (two), and 10 (1) e) How many genes have been identified that are required for the infection process of Puccinia antirrhini fungus? four

R...