HOL - Instance - Lab safety PDF

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  PHSL 1010 Physiology EXPLORATION 

EXPERIMENTATION 

Laboratory Safety EVALUATION 

Final Report Student Name

Neha Gupta

Institution

University of New England

Course

PHSL 1010 Physiology

Student ID Lesson

49340 Laboratory Safety

Session

Spring 2020

Instructor

Brandy Ferrara



Closely review your work. You will NOT be able to change your answers in the experimentation section once you click “Submit Your Data” below. This Final Report page displays the answers and data you recorded throughout the lesson. Review the entire page to ensure your work is accurate and nothing is missing. To finalize the report, click “Submit Your Data” below. Turn in the Final Report using your instructor’s preferred method.

Test Your Knowledge

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1. Match each term with the best description.

Tightly woven fabric used to smother and extinguish a fire

1

Fire blanket

2

Consists of absorbent material that can be ringed around a chemical spill until the spill can be neutralized

2

Spill containment kit

2

Device used to control small fires in an emergency situation

3

Fire extinguisher

2

Provides chemical, physical, health, and safety information regarding chemical reagents and supplies

4

Safety Data Sheet

2

Area containing an exhaust fan to expel noxious fumes from a laboratory

5

Fume hood

2

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2. Label each image with the correct term.

1

Safety shower

2

4

2

Eye-wash station 2

Burner fuel

2

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3

First aid kit

2

Fire extinguisher 2

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3. Categorize each statement as true or false.

True

False

1

2 Paper towels and water are a good substitute for a spill containment kit. Neutralize acid spills using sodium bicarbonate.

2

2

Induce vomiting and drink copious amounts of water if chemicals are ingested. Conduct experiments in a small, closedoff room, with no ventilation or water.

Always wear safety goggles, long shirts and pants, closed-toe shoes, and gloves when conducting experiments.

2

2

2

Exploration

1. For the most part, items in the lab kit are safe for use around children and pets. True False

2

FEEDBACK

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Right! Items should never be in reach of children or pets.

2. The laboratory kit contains _____. small items that can be choking hazards chemicals that can cause burns sharp objects that can cause cuts All of the above

2

FEEDBACK Great! https://myhol.holscience.com/instances/8102#/experimentation/106209/final

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 3. Eating, drinking, and smoking during an experiment are never allowed. True

2

False

FEEDBACK

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Great!

4. If any chemical in the kit has been ingested, you should _____. call the Poison Control Center seek a physician both call the Poison Control Center and seek a physician

2

FEEDBACK

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Good job!

5. After using loops, swabs, toothpicks, spreaders or any other equipment to transfer cultures, immerse the contaminated equipment in ______. pure bleach

2

a strong acid tap water All of the above

1

FEEDBACK

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After using loops, swabs, toothpicks, spreaders or any other equipment to transfer cultures, immerse the contaminated equipment in pure bleach.

6. Burns can occur when skin comes in contact with _____. acids bases acids and bases

2

FEEDBACK https://myhol.holscience.com/instances/8102#/experimentation/106209/final

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Excellent!

 7. To test the smell of a substance, partially fill your lungs with air and, while standing slightly back from the fumes, use your hand to waft the odors gently toward your nose. True

2

False

FEEDBACK

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Good work!

8. Most pieces of glassware are designed to be heated. True False

2

FEEDBACK

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Right! Only heatproof glassware is designed to be heated.

9. To better protect the body from chemical spills, wear _____. long-sleeved shirts full-length pants closed-toe shoes All of the above

2

FEEDBACK

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Nicely done!

10. When creating a home-laboratory, there are substitutions for the safety equipment found in an on-campus laboratory. True

2

False

FEEDBACK Yes! https://myhol.holscience.com/instances/8102#/experimentation/106209/final

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 11. At home, a _____ is the best substitute for a laboratory safety shower. water can bottle of water kitchen faucet bathroom shower

2

FEEDBACK

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Excellent!

12. A Safety Data Sheet contains information about first-aid procedures, required protective equipment, and health effects. True

2

False

FEEDBACK

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Great!

Exercise 1 Data Table 1: Lab Safety Equipment Alternatives

Shower or Sink

Paper Towels

Well-Ventilated Area

No Substitution

laboratory shower

spill containment kit

fume hood

first aid kit

eyewash station

Fire Extinguisher

safety goggles

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Exercise 2 Data Table 2: Sodium Hypochlorite SDS information

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Items

SDS Information

Physical State

liquid

Route of Exposure & Symptoms

eye contact, skin contact, inhalation, and ingestion can cause burning and irritation

Protective Equipment

As in any fire, wear selfcontained breathing apparatus pressuredemand, MSHA/NIOSH (approved or equivalent) and full protective gear.

First Aid Procedures

Get medical help immediately. Some other things that can be done are flushing eyes for 15 min, flushing skin with water for at least 15 min, giving 2-4 cups of milk or water, and give artificial respirations/ oxygen as needed.

Fire-fighting Measures

To extinguish media use water spray, dry chemical, carbon dioxide, or appropriate foam.

Chemical Reactivity

Bleach solutions decompose slowly at normal temperatures releasing low concentrations of corrosive chlorine gas. Decomposition is influenced by temperature, concentration, pH, ionic strength, exposure to light, and the presence of metals.

Safe Storage

Store in a tightly closed container and in a cool, dry, well-ventilated area away from incompatible substances.

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Safe Disposal

Bleach can be poured down the dink by small amounts as long as the bleach is diluted by the running water.

Environmental/ Ecotoxicity

No data/information is available

Spill Cleanup Procedures

Absorb spill using an absorbent, noncombustible material such as earth, sand, or vermiculite. Also provide ventilation

Exercise 3

1. What are small-scale techniques? Very small quantities of chemicals to protect ones safety during experiments at home.

2. List 5 precautions that must be taken before beginning an experiment. 1. Protect work table/surface by covering it with plastic sheeting or a white garbage bag 2. Have an open window or stove exhaust fan to provide proper ventilation 3. Have accessibility to water nearby (sink, bottled water, or bathroom shower) 4. Have a first aid kit nearby 5. Keep sodium bicarbonate (baking soda) on hand to neutralize spilled assets because the concentrated acids can cause serious burns

3. As indicated in the video, what common substance can be used to neutralize a spilled acid? sodium bicarbonate (baking soda).

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4. According to the video, why should a used chemical container never be refilled? Used chemical containers can be contaminated and an unexpected and potentially unpleasant reaction can occur.

5. Certain glass objects are not meant to be heated and could shatter if exposed to a heat source. What two examples of heat-sensitive glassware are given in the video? Two examples of heat-sensitive glassware are graduated cylinders and flasks, and can shatter when exposed to heat source.

Exercise 4 Data Table 3: Part 1 and Part 2 of the Safety Contract

Part

Agreement

Part 1: It is impossible to control students’ use of HOL Science products, related kits, and I agree: students’ work environments. The author(s) of HOL Science content, the instructors and ng institutions that adopt it, and Hands-On Labs, Inc. - the publisher and producer of HOL Science - authorize the use of these educational products only on the express condition that the purchasers and users accept full and complete responsibility for all and any liability related to their use of same. Please review this document several times until you are certain you understand and will fully abide by its terms. Part 2: I am a responsible adult who has read, understands, and agrees to fully abide by all I agree: ng safety precautions prescribed in HOL Science labs for laboratory work and for the use of HOL Science kits. Accordingly, I recognize the inherent hazards associated with science experimentation; I will always experiment in a safe and prudent manner; and I unconditionally accept full and complete responsibility for any and all liability related to my purchase and/or use of a science HOL Science kit or any other science products or materials provided by Hands-On Labs, INC.

Competency Review

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1. Chemicals in a lab kit may potentially cause _____ if mishandled or consumed. burns serious illness death All of the above

2

FEEDBACK

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This question focuses on Learning Objective 1: Discuss basic safety concerns and guidelines.

2. When conducting experiments that include vigorous exercise, consult a physician first or ask a partner to assist in participating if you are unable to do so. True

2

False

FEEDBACK

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This question focuses on Learning Objective 1: Discuss basic safety concerns and guidelines.

3. The _____ should be contacted immediately if you or anyone accidentally consumes or comes in contact with a potentially toxic substance. National Chemical Toxicity Center National Poison Control Center

2

American Poison Chemical Society American Toxic Chemical Center

FEEDBACK



This question focuses on Learning Objective 1: Discuss basic safety concerns and guidelines.

4. At the conclusion of experiments involving growing bacterial cultures, _____ should be added to the plates or tubes and everything should be soaked for _____. pure acid; 1 hour a base; 30 minutes pure bleach; 2 hours

2

pure bleach; 15 minutes

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FEEDBACK



This question focuses on Learning Objective 4: Compare safe practices in traditional and home laboratories.

5. When working with acids, always _____ to avoid chemical splattering. add acid to water

2

add water to acid

FEEDBACK

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This question focuses on Learning Objective 2: Identify potential laboratory hazards.

6. Acid spills can be neutralized by adding _____. water baking soda

2

more acid distilled water All of the above

FEEDBACK



This question focuses on Learning Objective 4: Compare safe practices in traditional and home laboratories.

7. Graduated cylinders and volumetric flasks are designed to be heated. True False

2

FEEDBACK

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Graduated cylinders and volumetric flasks are not designed to be heated. Heating graduated cylinders and flasks may cause them to burst and shatter. This question focuses on Learning Objective 2: Identify potential laboratory hazards.

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8. A _____ can be used to douse a fire. fire extinguisher safety shower fire blanket fire blanket and fire extinguisher All of the above

2

FEEDBACK



This question focuses on Learning Objective 3: Describe various forms and uses of safety equipment.

9. Chemicals may be returned to their containers after they have been dispensed. True False

2

FEEDBACK

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Do not return chemicals to their containers after they have been dispensed to prevent contamination. This question focuses on Learning Objective 4: Compare safe practices in traditional and home laboratories.

10. Normal eyeglasses may be worn to substitute for safety goggles. True False

2

FEEDBACK

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Normal eyeglasses provide minimal protection as they are not enclosed around the eyes like safety goggles are. There is no safer alternative to safety goggles. This question focuses on Learning Objective 3: Describe various forms and uses of safety equipment.

11. A _____ expels noxious fumes from a laboratory. fire blanket fume hood

2

spill containment kit All of the above https://myhol.holscience.com/instances/8102#/experimentation/106209/final

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FEEDBACK



This question focuses on Learning Objective 3: Describe various forms and uses of safety equipment.

12. A sink faucet or hand-held shower wand are appropriate substitutes for an eyewash station. True

2

False

FEEDBACK



This question focuses on Learning Objective 3: Describe various forms and uses of safety equipment.

13. A Safety Data Sheet provides basic information about _____. health effects toxicity physical data safe disposal health effects and toxicity All of the above

2

FEEDBACK

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This question focuses on Learning Objective 5: Outline components of a safety data sheet.

14. First-aid kits are best substituted by bandages and alcohol. True False

2

FEEDBACK

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There is no substitution for a first-aid kit. This question focuses on Learning Objective 3: Describe various forms and uses of safety equipment.

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Extension Questions

1. Use the Internet to look up the SDS for hydrochloric acid and answer the following questions: a. List the potential acute health effects. b. List the potential chronic health effects. c. Identify the first aid measures for ingestion. d. Record the flammability of the product. e. Identify 3 chemicals that produce a potentially dangerous reaction with hydrochloric acid. f. Describe how to handle small spills. g. List the personal protection when working with the chemical. a. List the potential acute health effects. Eye: irreversible eye injury, irritation, and severe burns Skin: severe burns and ulceration Ingestion: severe digestive tract burns with abdominal pain, vomiting, and possible death. It could also cause corrosion and permanent tissue damage to the esophagus and digestive tract Inhalation: Severe irritation to the respiratory tract with symptoms like sore throat, coughing, SOB, delayed lung edema, and corrosive action to mucous membranes b. List the potential chronic health effects. Dermatitis Erosion of the teeth Bleeding of nose and gums Chronic bronchitis Chronic gastritis

c. Identify the first aid measures for ingestion. Rise mouth Do not induce vomiting drink sips of water and seek medical attention immediately

()

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