Lab 9 ans. - microbiology lab 9 PDF

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microbiology lab 9...

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Differential and Biochemical Tests 1.

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Differential and Biochemical Tests EXPERIMENT 2: CATALASE ASSAY Data Tables Table 2: Experiment 2 Assay Results Petri Dish Section

Colon y

Bubbles? (Yes or No)

Catalase Positive or Negative?

Sink

1

Yes

Positive

Sink

2

Yes

Positive

Shoe

1

Yes

Positive

Shoe

2

Yes

Positive

Phone

1

No

Negative

Phone

2

Yes

Positive

Post-Lab Questions 1. What are the possible types of respiration that catalase-positive samples might be capable of? Catalase-negative? The positive catalase uses aerobic breathing, whereas the negative catalase uses aerobic breathing.

2. Did the colonies from each plate show the same reaction? Why or why not? How can you interpret this? The samples displayed various reactions from the phone. However, most of the colonies responded the same. Samples are produced of various bacteria, including aerobic and anaerobic microbe.

3. Research and discuss a scenario in a lab or clinical setting in which you would need to perform a catalase test. In a laboratory, catalase studies are used to differentiate Clostridium from Bacillus strains because the Clostridium strains are catalase-negative and because the Bacillus strains are positive.

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Differential and Biochemical Tests 4. What reaction would you expect when performing a positive control in the catalase assay? What would it mean if a known catalase-positive bacterium did not produce the expected reaction?

I will predict bubbles from optimistic regulation automatically. If an established catalasepositive bacterium does not create bubbles, I realize that the test is ineffective, and that new test materials must be checked or used.

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Differential and Biochemical Tests EXPERIMENT 3: ANTIBIOTIC SENSITIVITY Data Tables Table 3: Zone of Inhibition for Skin Sample Antibiotic Disk

Diameter of Zone (mm)

AM10

6

K30

13

Te30

4

Table 4: Zone of Inhibition for Local Sample Antibiotic Disk

Diameter of Zone (mm)

AM10

0

K30

9

Te30

3

Table 5: Zone of Inhibition for Yeast Sample Antibiotic Disk

Diameter of Zone (mm)

AM10

0

K30

10

Te30

8

Post-Lab Questions 1. Table 6 outlines interpretive standards of antibiotic resistance based on the size of inhibition zones. For example, many bacterial species that are resistant to Kanamycin have an inhibition zone of 13 mm or smaller. Of course, inhibition zones may also be affected by the antibiotic concentration present within the disk. Use Table 6 to determine whether the microbial samples you tested are sensitive (S), moderately resistant (MR), or resistant (R) to each of the antibiotic disks. Remember, you will not know the exact

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Differential and Biochemical Tests species of bacteria on your “Skin” and “Local” plates. The table is a guide to compare your results to a standard.

The isolated bacterial after three days of development in Mueller-Hinton Agar Plates Multiple antibiotics were found to have distinct areas of inhibition. The Regions Skin samples are inhibited with Ampicillin 6 mm, indicating bacteria seen in isolation Table 6 resistance; Kanamycin inhibition zone was 13 mm, That the antibiotics were immune to the bacteria based on table 6; Tetracycline was blocked at 4 mm, which indicates the bacteria are immune to Antibiotic. Antibiotic. Ampicillin 0 mm is the inhibition region for the local sample; The resistance of the isolated bacteria is based on the findings of table 6. The inhibition zone for Kanamycin could be a species Staphylococcus; that the bacteria are also antibiotic-resistant; Tetracycline inhibition zone 3 mm, which indicates that the bacteria are often antibiotic tolerant. Ampicillin 0 mm is an inhibitory region for the yeast extract. Ampicillin defied the yeast; Kanamycin inhibition region was 10 mm, means that the region of inhibition of tetracycline was also immune to kanamycin 8 mm, that was to suggest that Tetracycline was also immune to the yeast.

2. Is the bacterial lawn sampled from your skin more resistant to one antibiotic than the others? What about the local sample? The yeast sample? Use your results from Question 1 to support your answer.

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Differential and Biochemical Tests While all three separate samples of multiple bacterial sources (skin, Resistance to three antibiotics to which they were subjected during 3 was seen local, yeast) Days, each antibiotic displayed various inhibition areas, which increased Immune of most citizens. For instance, the skin's bacterial lawn sample Higher Tetracycline resistance (inhibition region 4mm) than Kanamycin (inhibition zone 13mm) and ampicillin (inhibition zone 6mm). The local sample was available—quite close skin sample findings. Kanamycin was the bacterial antibiotic. More sensitivity showed a 9 mm inhibition region, while Tetracycline saw 3 mm, And Ampicillin had no inhibition region whatsoever. Kanamycin in the yeast sample the bacteria again prevailed and showed more susceptibility to this antibiotic (zone). 10 mm inhibition of Tetracycline (inhibition region 8 mm) and Ampicillin (Inhibition region 0 mm)

3. What is the significance of the size of the zone of inhibition with respect to the microbe samples?

The scale of the inhibition region shows the exposure of the bacteria to a certain compound. The smaller, the bigger Bacterial exposure to the compound would be higher in the region of inhibition.

4. Research and describe the relationship between the minimum inhibitory concentration (MIC) and the zone of inhibition. What can you determine about the MIC and the effectiveness of the antibiotics used in this experiment?

Note, disk potencies are listed per the findings of the Kirby-Bauer test are generally recorded in the field of inhibition as susceptible, intermediate, or immune; the minimum inhibitor concentration is the degree of antibiotically sufficient to stop bacterial growth in

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Differential and Biochemical Tests a given crop. The greater the inhibition region, the smaller the concentration of a certain antibiotic would be required to inhibit certain bacteria's growth or living (Norman-McKay, 2019). The MIC was very low to discourage the development of bacteria. Tetracycline and Kanamycin were also of similar ability (30 ugs), but the inhibition region was greater than Kanamycin. According to the Kanamycin MIC, a lower drug level than Tetracycline is required for the growth of some bacteria. antibiotic in the Materials section before the experimental procedure.

5. Use a reputable internet source to determine and describe which cellular component in the bacterial cell is targeted by each of the antibiotics used in this experiment.

Ampicillin attaches and inactivates penicillin-bound proteins on the internal membrane of the cell wall of the bacteria. The inactivation of these proteins impedes peptidoglycan chains that preserve a solid and stiff cell wall. Ampicillin also disrupts the synthesis of bacterial cell walls and leads to lysis and further cell mortality in the cell walls (Kozier & Hodgson, 2018). Kanamycin acts by attaching the ribosomal subunit in the bacterial 30s allowing the t-RNA to lose the reading that helps the bacteria further synthesize proteins important to their growth (Kozier &Hodgson 2018). The ribosome's A-site, altering cytoplasm membrane and additional leakage of intracellular material, which holds the cell intact, is stopped by the amino-acyl tRNA (Kozier & Hodgson, 2018).

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