LAB REPORT BIO 15O PDF

Preview

Summary

LAB REPORT BIOLOGYEXPERIMENT I: ENZYME IEXPERIMENT II: ENZYME IIOBJECTIVETo determine the reasons influence the reaction of catalase and analyse the reaction of catalase on sort of samples.INTRODUCTIONHydrogen peroxide breaks down into water and hydrogen by the enzyme catalase. Hydrogen peroxide is ...

Description

LAB REPORT BIOLOGY EXPERIMENT I: ENZYME I EXPERIMENT II: ENZYME II

OBJECTIVE To determine the reasons influence the reaction of catalase and analyse the reaction of catalase on sort of samples.

INTRODUCTION Hydrogen peroxide breaks down into water and hydrogen by the enzyme catalase. Hydrogen peroxide is active chemical formed as a by-product of chemical reaction in living cells and it will destroy if it were not immediately broken down by cells. Catalase 2H2O2

2H2O2 + O2

MATERIALS a) 7 test tubes b) Pestle and mortar c) Beaker 250cm3 d) Thermometer e) Bunsen burner f) Hydrogen peroxide g) Liver h) Kidney i) Potato

PROCEDURE a) 2 cm3 hydrogen peroxide solutions has pour into a clean tube. 1cm cube of liver has cut and then drop it into the test tube of hydrogen peroxide. The reaction has observed and recorded carefully. Lastly, the tube tested for oxygen with a splint. b) Another 1cm cube of liver has placed in a mortar. The liver has grinded and the ground up liver transferred into a test tube containing a hydrogen peroxide. The

reaction of the ground of liver has compares with the reaction for the whole piece of liver has observed. c) Another piece of liver has put it in a beaker of boiling water for three minutes. Then the liver has dropped into the hydrogen peroxide and the capability of enzyme of breaking down the hydrogen peroxide has observed. d) The experiment has repeated again but using kidney and potato and the presence of enzyme catalase is recorded. e) Observation of experiment has recorded in Table 1.1.

QUESTION 1. As the temperature and the substrate concentration increases, the rate of catalase reaction will go up too. 2. As the temperature increases toword the optimum point, hydrogen bond loosen, making it easier for catalase to act on the hydrogen peroxide molecules. If the temperature increases beyond the optimum point, the enzyme denatures, and its structure is distruped . 3. In the first part of this experiment we prepared 3 catalase which are liver, kidney and potato and put these catalase in the three difference test tube that contains hydrogen peroxide.We notice that the first test tube with liver the observation when we put the liver into the test tube it bubble up fastest and there oxygen present.Next is the observation of kidney, the bubbles seem to bubbles up slowly and it also has the oxygen present.Lastly, we test it using potato and the result seems that the bubbles up more slowly than the two catalase but the oxygen still present . CONCLUSION Enzymes are very efficient catalysts for biochemical reactions. These biological catalysts increase the rate of the reaction when exposed to the optimun temperature and would underperfom when the temperature lower and or higher than equal optimal temperature ....