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Cell Staining and Microscopy Lab Worksheet Size Estimation Directions: Complete Table 1 to determine the field of view (FOV) for each objective lens (10X, 40X, and 100X). Table 1

Estimation of Magnification Power of a Compound Microscope Magnification

Power of Lens

FOV Area (mm2)

Diameter (µm)

FOV Diameter (mm)

4X

40x

4.5mm

4,500

10X

100x

1.8mm

1,800

2.83

40X

400

.45mm

450

.71

1000x

.18mm

180

.28

100X

7.07

Observation of Prepared Specimens Directions 1. Observe the indicated specimen (paramecium, volvox, and ameoba) at the optimal magnification. Optimal magnification is the magnification that allows the entire organism to be seen in the greatest detail. 2. Note the lens of observation and the total magnification. 3. Using the information from Table 1, estimate the size of the specimen at this magnification.

Specimen: Paramecium Lens of observation: 40x Total magnification: 400x Estimated size of Paramecium: 1575 µm (Remember to include the units of metric measure) Sketch the organism that was observed under the microscope.

1

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Cell Staining and Microscopy Lab Worksheet Specimen: Volvox Lens of observation: 40x Total magnification: 400x Estimated size of Volvox: 2,700 µm (Remember to include the units of metric measure) Sketch the organism that was observed under the microscope.

Specimen: Amoeba Lens of observation: 40x Total magnification: 400x Estimated size of Amoeba: 2,250 µm (Remember to include the units of metric measure) Sketch the organism that was observed under the microscope.

Amoeba was pink

Isolation and Staining of Cheek Epithelial Cells Lens of observation: 40x Total magnification: 400x Estimated size of epithelial cheek cells: __________________ (Remember to include the units of metric measure) Sketch an image of the epithelial cells observed under the high-power lens. `

Review Questions Answer each question using full sentences. 1. Paramecium was found to occupy 20% of the FOV of a microscope at 40X magnification power. How many paramecia can be accommodated within this FOV?

2.25

2. If 300 Amoeba can be contained within a 50 mm2 area of the FOV of the microscope, what would the approximate size of each parasite be? (Note: A=πr^2)

50/300 = .16mm

3. Plasmodium was found to occupy 2% of the FOV of a microscope with 100 mm diameter. What would the size of the parasite be? How many parasites can be accommodated within this FOV? .02*100=2

4. A worm is 2 mm in size and occupies 60% of the FOV. What is the diameter of the FOV at this unknown magnification? 2/.6= 3.33mm

5. What is the diameter of a FOV at an unknown magnification if the size of a flea is 2 mm, and it occupies 30% of the FOV? 2/.3=6.67 mm

6. If the diameter of the FOV of a compound microscope at 40X magnification is 450 micrometers (µm), what would be the FOV at 400X magnification? Dh/.45=4/400

7. A parasite occupies 30% of the FOV in a microscope under 400X total magnification. At this magnification, the FOV of the microscope is 200 µm. What is the size of the parasite? 1.5mm...