Lab5 - Jupyter Notebook PDF

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10/28/2020

Lab5 - Jupyter Notebook

Lab 5 Activity B: Testing the Antimicrobial Properties of Spices (16 Marks) In past years your lab was completed with your group members. While you may discuss the assignment with the other students in your lab and consult the lab notes, be careful not to plagiarize - your answers MUST be in your own words. Normally you would analyze the data from your entire practical section. This year, the data for your analysis are provided in the Lab5 folder. The data are in the plain text file called lab5.activityB.data.txt . When you are done the assignment you can download your notebook as a PDF and submit it on the BIO152 course page.

Experimental Question: Does garlic affect bacterial growth? ¶ Question 1: What is the independent variable? (1 mark) Then independant variable it garlic concentration

Question 2: What is the dependent variable? (1 mark) The dependent variable is amount of bacteria growth

Question 3: Formal Hypothesis Statement: (1 mark) The garlic concentration will affect the amount of bacteria growth

Question 4: What do you think should happen to bacterial growth in the garlic treatments? Why? (1 mark) I think that there will be less bacterial growth in the garlic treatements and that the higher the concentration of garlic, the less bacteria will grow. I think this because garlic is proposed to be an antimicrobial agent. This is proposed because plants and spices are phytochemicals, which can inhibit bacteria growth on the plants themselves

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Lab5 - Jupyter Notebook

Question 5: Do you expect to see bacterial growth around the negative control disc when you examine your plates? Why/why not? (1 mark) I do expect to see some bacterial growth around the negative control disc because there was no antimicrobial agent added to the negative control meaning that there is no reason for the ecoli to not grow.

Question 6: What would a ZOI around the control disc tell you about the validity of your experimental results? Explain. (2 marks) If there is a ZOI around the control it would mean that the experiment was somewhat compromised and the experimental results were not valid as there was no antimicrobial agent added and therefore no reason for there to be an ZOI

Question 7: Do you expect to see bacterial growth around the positive control disk (streptomycin) when you examine your plates? Why or Why not? (1 mark) I do not expect to see any bacteria around the positive control disc since I expect it to have a ZOI. This is because streptomycin is a known antimicrobial agent and therefore should inhibit the bacterial growth arouf the disc.

Results

R Exercise: Read in data (1 Mark) In the Lab5 folder there is a data file called lab5.activityB.data.txt Read the file into a data frame and assign the data frame to an object called lab5_df This table includes two columns: 1. ZOI : The diameter (in mm) of the Zone of Inhibition measured 2. Treatment : The treatment for each ZOI measurement. Treatment can take four values: NegControl: Negative Control, Water PosControl: Positive Control, Streptomycin Garlic1: Garlic suspension @ 1X concentration Garlic2: Garlic suspension @ 0.5X concentration https://biojupyterhub.utm.utoronto.ca/user/larrond1/notebooks/Lab5/Lab5.ipynb#

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Lab5 - Jupyter Notebook

https://biojupyterhub.utm.utoronto.ca/user/larrond1/notebooks/Lab5/Lab5.ipynb#

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Lab5 - Jupyter Notebook

 lab5_df...