Lecture Notes: Dan Luo Guest Lecture PDF

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Lecture notes for Prof. Dan Luo's guest lecture. ...

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Dan Luo Lecture  

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DNA is both a genetic and a generic material (has tunable length) DNA possesses amazing properties o Mechanical  dsDNA: persistence length = 50 nm  flexible or regid  plexiglass o Physical  Small  Programmable  Charged o Chemical  Stable  Biodegradable/sacrificable  Non-toxic, water soluble, monodisperse  Can be synthesized in a large quantity and high purity o DNA are highly modifiable via self assembly (programmable) and post modifications DNA can be programed o Palindromic sticky end  Molecule recognizes itself, becomes twice as long o Non-palindromic sticky end  Change one base in palindrome, bases don’t stick together Luo’s research o DNA barcode  Dendritic DNA  Made Y, and then made Y’s on Y’s  Dendritic DNA can be used for barcoding (detecting different color ratios rather than different colors o Hydrogel  Can throw gene into the fabric of the matrix and conjucate them to x matrices  Can transcribe those genes in situ to make proteins within the matrix o Nanoparticle DNA versus synthetic polymer o Topology of DNA polymers were almost always linear; first research was to try to branch DNA o Branched DNA: Engineering design barameters  Must be in bulk scale, robust, precisely controllable, and high in yield/efficiency o Dendritic DNA  Made Y, and then made Y’s on Y’s

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Dendritic DNA can be used for barcoding (detecting different color ratios rather than different colors...