Lecture notes – The overview of Grocott-Gomori’s Methenamine Silver Staining PDF

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Lecture notes – The overview of Grocott-Gomori’s Methenamine Silver Staining The process of Grocott-Gomori’s Methenamine Silver (GMS) stain is a histological stain that is used majorly for the identification of carbohydrates in fungal microorganisms. This staining method was named after György Gömöri, a physician from Hungary, who developed the staining methodology. This means Its initial application to assess missing tissues and diseases in the liver and the rectum (Nadworny, Wang, Tredget, & Robert, 2010) and then used for the identification of Pneumocystis jiroveci, a fungus known to cause an opportunistic infection called pneumocytosis, in immunocompromised and immunosuppressed patients. This is In comparison to other stains like Periodic Acid-Schiffs stain and Gridley Stain, Goromi has a higher sensitivity to for detecting fungi and other polysaccharide-rich microorganisms in paraffin prepared sections. The Gomori’s methenamine-silver nitrate and chromic acid comprise the major reagents used in conventional Grocott stain It has also been used for the identification of fungi in tissue sections. Its application in Histology makes it ideal for the detection and demonstration of fungi in aspirates, tissues, and smears.

The differen Objectives To demonstrate the presence of fungi in a given sample. To demonstrate the presence of Pneumocystis jiroveci and Histoplasma spp.

freestar Principle of Grocott-Gomori’s Methenamine Silver Staining The fungal cell wall is composed of polysaccharides which on interaction with chromatin Acid, undergo oxidation to form aldehydes. This is demonstrated by the reduction of the alkalinehexamine-silver complex.

The process of Grocott’s alkaline hexamine-silver solution undergoes reduction to form precipitates of silver ions making the cell wall of the fungi appear black a reaction is known as argentaffin reaction (the ability of cells to reduce the silver solution to metallic silver forming a black tissue element). Argentaffin cells are found of the epithelial lining of the lungs, the intestines, and melanin. This reaction drives the outcome of the result of the stain.

The staining methodologies are of two types:

The conventional method at room temperature Microwave method

The Reagents Chromium trioxide solution, Sodium Bisulfite solution, Silver nitrate solution, methenamine solution, Borax solution, Gold chloride solution, sodium thiosulfate solution, Light green stock solution....