MIC456 Practical 2 lab manual and questions PDF

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Practical 2: Media Preparation and Bacterial cultivationPlease watch the following videos: youtube/watch?v=fzk_-O2SDos – Preparation of culture media youtube/watch?v=0dlDf8HiiQw – Autoclave youtube/watch?v=VjCbH0Ipkco – Correct labelling of plates youtube/watch?v=6gFOAIhPaOs&t=41s – Pouring agar...

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Practical 2: Media Preparation and Bacterial cultivation

Please watch the following videos:

1. 2. 3. 4. 5. 6. 7.

https://www.youtube.com/watch?v=fzk_-O2SDos – Preparation of culture media https://www.youtube.com/watch?v=0dlDf8HiiQw – Autoclave https://www.youtube.com/watch?v=VjCbH0Ipkco – Correct labelling of plates https://www.youtube.com/watch?v=6gFOAIhPaOs&t=41s – Pouring agar media https://www.youtube.com/watch?v=DbRzNgoIF7s – Single colony streaking https://www.youtube.com/watch?v=HfaYPct50Ks – single colony streaking (2) https://www.youtube.com/watch?v=4JZAFUPckUg – colony morphology

Now, let’s say you have done your practical as shown in the videos above. After incubating your Petri dishes of single colony streaking, you got the results as shown below:

Plate 1

Plate 2

Plate 3

With the given results, write a lab report and answer the questions below. 1. The manufacturer’s instructions stated that to prepare a 1 L of the media, you need to add 14.65 g of the powdered formula. If your lecturer asked you to prepare a 200 ml of the media, calculate how much of the powdered formula would you need. 2. What are some common mistakes that can be made in single colony streaking that would make it unsuccessful? 3. Which of the three plates was unsuccessful and what error could have cause it to be unsuccessful? 4. Why is single colony streaking important in microbiology?...