Micro lab- Worksheet 1 PDF

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Micro lab- Worksheet 1...

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Bio 254 Lab: Microbial Metabo Experiment No. Name of biochemical test

Ex 21

Ex 22

Extracellular enzymes:

Carbohydrate Fermentation

Starch hydrolysis Lipid hydrolysis Casein hydrolysis

Test Medium Used

Gelatin hydrolysis Starch Agar Plate Spirit Blue Agar Plate Skim Milk Agar Plate Nutrient Gelatin Deep Tube

O

i

U d

E h i hi

Phenol Red Lactose Broth Tube w/ Durham Tube Phenol Red Sucrose Broth Tube w/ Durham Tube Phenol Red Glucose Broth

li

Tube w/ Durham Tube SUGAR

Enzyme tested

Does the test require a reagent to be added? (name of reagent/how many drops?) Expected Positive Results What changes are expected in Medium? End products

Amylase

Glucose

Lipases

Sucrose

Caseinase Proteases

Lactose

Gelatinase Yes- Gram’s iodine solution

Starch hydrolysis Positive result: If the starch has been hydrolyzed, a clear zone of hydrolysis will surround the growth of the organism Lipid hydrolysis Loss of opacity is the result of the hydrolytic reaction yielding soluble glycerol and fatty acids indicating a

Phenol red

After incubation, carbohydrates that have been fermented with the production if acidic wastes will cause the phenol red (pH 7) to turn yellow (slightly acidic pH (6.8) indicating a positive reaction In some cases, acid production is accompanied by the evolution of a gas (CO2) that will be visible as a

Solidification represents negative reaction...