OL Biosafety-1 - biosafety worksheet for micro PDF

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biosafety worksheet for micro...

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BIOS242, Week 7-OL Name:

Lab 7: Biosafety Learning Outcomes    

Explain how a biosafety containment level III laboratory is constructed (e.g. air flow and pressure control). Explain the basic safety rules of a biosafety containment level III laboratory (e.g. use of safety equipment). Handle microorganisms in a biosafety containment level III laboratory. Explain the concept of fumigation and how this is executed within a microbiological safety cabinet.

Introduction: Experiment with biosafety virtual simulation lab In the Biosafety simulation, you will be introduced to the concept of laboratory containment, which is a very special type of laboratory required to work with hazardous pathogens. Laboratory containment Containment in this context refers to the requirements set out by various bodies to safely manipulate such pathogens. In this simulation, you will be able to experience a containment level 3 (Biosafety Level 3 or BSL3) research laboratory. Learn about lab safety You will become familiar with the basic protocols utilized to ensure safety, and you will be presented with the specific basic safety rules for this type of laboratory (e.g. personal protective equipment (PPE) and primary containment). In addition, you will learn how every part of the laboratory is designed to avoid contamination. This includes learning about why the pressure in the laboratory needs to be controlled at all times (secondary containment), and how the microbiological safety cabinets are designed to offer protection). Identify the hazard In the biosafety simulation you will be presented with a hypothetical case where you will need to identify a potential bioterrorism agent that is classed as a hazard group three microorganism. Please note that this simulation has been developed broadly around UK regulations. Some protocols may differ depending on the specific regulations. Questions: 1. Purpose: Please describe in complete sentences and in your own words, the purpose of this experiment.

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BIOS242, Week 7-OL Name:

The purpose of this experiment was to become familiar with the requirements for different levels of a laboratory and what types handle different levels of pathogens . It taught me about the use of pressure and air needed in order to avoid a pathogen from escaping from the lab setting and making its way into the regular world. 2. What are the different biosafety levels? BSL-1, BSL-2, BSL-3, and BSL-4

3. What practices would you recommend to be used in BSL 1 and how do these differ from BSL 2? BSL 1 is when you are working with a low level microbe that does not stand a chance of causing any serious or no harm to a healthy person. You can expect to work with mechanical pipetting, safe handling of sharps and hand washing . BSL 2 is involved with microbes that can cause a moderate health hazard. You are expected to wear PPE, the lab will have self-closing doors and procedures that can cause infection from aerosols or splashes are performed within a biological safety cabinet

4. In this simulation what organism was discussed? Which biosafety level is this pathogen handled in? Why? Organism discussed was anthrax. It is handled in a BSL-3 setting in order to avoid and prevent any serious implications.

5. What does biosafety level 4 mean? What type of pathogens can only be handled in these labs? Biosafety level is the highest level of biosafety precautions. It is appropriate for work with agents that could easily be aerosol-transmitted within the laboratory and cause severe to fatal

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BIOS242, Week 7-OL Name:

disease in humans for which there are no available vaccines or treatments. Examples of pathogens handled includes s Marburg virus, Ebola virus, Lassa virus, and Crimean-Congo hemorrhagic fever, Hendra virus and Nipah virus .

6. Reflection: Write 5 sentences on what you learned from this simulation. What did you like and what was something that you would prefer not be a part of this simulation?

I learned how to operate a biosafety containment cabinet. I learned what to do and how to handle a spill. I learned about double gloving and cleaning with bio cleanse at the end of the day. I also learned about the importance of pressure in the lab.

I liked that this stimulation allowed me to understand how to work in a lab setting with a different set up precautions in place when compared to a standard lab. I disliked that the labster takes forever to load every week and that the stimulation is often very slow and I have to sit there staring at the screen most of the time .

Grading Rubric: Activity Lab Report and Questions

Deliverable Complete answer questions 

Points 15

Purpose (2 point) 3

BIOS242, Week 7-OL Name:

 All Lab Deliverables

Questions (8 points)

 Reflection (5 points) Complete ALL lab work and lab report

15

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